FuBP1 recombinant protein

FuBP1, 279-448aa, Human, His tag, E Coli

Cat. Num. AAA204764

• Gene Names: FUBP1; FBP; FUBP; hDH V
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: FuBP1; 279-448aa; Human; His tag; E Coli; Far upstream element (FuSE) binding protein 1; FBP; FuBP; FuBP1 recombinant protein

• Host: E Coli
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol, 0.15M NaCl
• Concentration: 0.5 mg/ml (determined by BCA assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMDVPIP RFAVGIVIGR NGEMIKKIQN DAGVRIQFKP DDGTTPERIA QITGPPDRCQ HAAEIITDLL RSVQAGNPGG PGPGGRGRGR GQGNWNMGPP GGLQEFNFIV PTGKTGLIIG KGGETIKSIS QQSGARIELQ RNPPPNADPN MKLFTIRGTP QQIDYARQLI EEKIG
• Sequence Length: 644

• Applicable Applications for FuBP1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for FuBP1 recombinant protein

FuBP1 is a ssDNA binding protein that activates the far upstream element (FuSE) of c-myc and stimulates expression of c-myc in undifferentiated cells. Regulation of FuSE by FuBP occurs through single-strand binding of FuBP to the non-coding strand. This protein has been shown to function as an ATP-dependent DNA helicase. Recombinant human FuBP1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for FuBP1 recombinant protein

Epigenomics

References

Zheng,Y., et al. (2011) Int. J. Biochem. Cell Biol. 43 (11), 1641-1648; Neubauer,A., et al. (2011) Exp. Hematol. 39 (10), 1030-1042.

NCBI and Uniprot Product Information

• NCBI GI #: 17402900
• NCBI GeneID: 8880
• NCBI Accession #: NP_003893
• NCBI GenBank Nucleotide #: NM_003902.4
• UniProt Accession #: Q96AE4; Q12828
• Molecular Weight: 20.8kDa (195aa) confirmed by MALDI-TOF
• NCBI Official Full Name: far upstream element-binding protein 1 isoform 2
• NCBI Official Synonym Full Names: far upstream element binding protein 1
• NCBI Official Symbol: FUBP1
• NCBI Official Synonym Symbols: FBP; FUBP; hDH V
• NCBI Protein Information: far upstream element-binding protein 1
• UniProt Protein Name: Far upstream element-binding protein 1
• Protein Family: Far upstream element-binding protein
• UniProt Gene Name: FUBP1
• UniProt Synonym Gene Names: FBP; FUSE-binding protein 1; hDH V

NCBI Description

The protein encoded by this gene is a single stranded DNA-binding protein that binds to multiple DNA elements, including the far upstream element (FUSE) located upstream of c-myc. Binding to FUSE occurs on the non-coding strand, and is important to the regulation of c-myc in undifferentiated cells. This protein contains three domains, an amphipathic helix N-terminal domain, a DNA-binding central domain, and a C-terminal transactivation domain that contains three tyrosine-rich motifs. The N-terminal domain is thought to repress the activity of the C-terminal domain. This protein is also thought to bind RNA, and contains 3'-5' helicase activity with in vitro activity on both DNA-DNA and RNA-RNA duplexes. Aberrant expression of this gene has been found in malignant tissues, and this gene is important to neural system and lung development. Binding of this protein to viral RNA is thought to play a role in several viral diseases, including hepatitis C and hand, foot and mouth disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Uniprot Description

Regulates MYC expression by binding to a single-stranded far-upstream element (FUSE) upstream of the MYC promoter. May act both as activator and repressor of transcription.