Canine Follicle Stimulating Hormone ELISA Kit | FSH elisa kit

Canine Follicle Stimulating Hormone ELISA Kit

Cat. Num. AAA46790

• Reactivity: Canine
• Synonyms: Follicle Stimulating Hormone; Canine Follicle Stimulating Hormone ELISA Kit; FSH elisa kit

• Reactivity: Canine
• Specificity: No significant cross-reactivity or interference between Canine FSH and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 0.625 mIU/ml - 20 mIU/ml.
• Sensitivity: 0.1 mIU/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated FSH concentrations in Canine serum, plasma and other body fluids. Using Purified Canine FSH antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add FSH and FSH antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of FSH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for FSH elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated FSH concentrations in Canine serum, plasma and other body fluids. Using Purified Canine FSH antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add FSH and FSH antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of FSH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 182762
• NCBI GeneID: 2488
• UniProt Accession #: P01225; Q14D61; A2TF08; A5JVV3
• Molecular Weight: 14,700 Da
• NCBI Official Full Name: follicle-stimulating hormone
• NCBI Official Synonym Full Names: follicle stimulating hormone, beta polypeptide
• NCBI Official Symbol: FSHB
• NCBI Protein Information: follitropin subunit beta; FSH-B; FSH-beta; follitropin beta chain; follitropin, beta chain; follicle-stimulating hormone beta subunit
• UniProt Protein Name: Follitropin subunit beta
• UniProt Gene Name: FSHB
• UniProt Synonym Gene Names: FSH-B; FSH-beta
• UniProt Entry Name: FSHB_HUMAN

NCBI Description

The pituitary glycoprotein hormone family includes follicle-stimulating hormone, luteinizing hormone, chorionic gonadotropin, and thyroid-stimulating hormone. All of these glycoproteins consist of an identical alpha subunit and a hormone-specific beta subunit. This gene encodes the beta subunit of follicle-stimulating hormone. In conjunction with luteinizing hormone, follicle-stimulating hormone induces egg and sperm production. Alternative splicing results in two transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

FSHB: Stimulates development of follicle and spermatogenesis in the reproductive organs. Defects in FSHB are a cause of isolated follicle- stimulating hormone deficiency (IFSHD). Selective follicle-stimulating hormone deficiency is an uncommon cause of infertility, producing amenorrhea and hypogonadism in women and oligo or azoospermia with normal testosterone levels in normally virilised men. Belongs to the glycoprotein hormones subunit beta family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 11p13

Cellular Component: cytoplasm; extracellular region

Molecular Function: follicle-stimulating hormone activity; protein binding; hormone activity

Biological Process: progesterone biosynthetic process; follicle-stimulating hormone signaling pathway; peptide hormone processing; female gamete generation; Sertoli cell proliferation; female pregnancy; signal transduction; regulation of osteoclast differentiation; cellular protein metabolic process; transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; positive regulation of bone resorption; positive regulation of transcription from RNA polymerase II promoter; positive regulation of cell migration

Disease: Follicle-stimulating Hormone Deficiency, Isolated