Fascin (FSCN) Recombinant Protein | FSCN recombinant protein

Recombinant Fascin (FSCN)

Cat. Num. AAA2011869

• Gene Names: FSCN1; HSN; SNL; p55; FAN1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Fascin (FSCN); Recombinant Fascin (FSCN); FSCN recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF- QGMD LSANQDEETD QETFQLEIDR DTKKCAFRTH TGKYWTLTAT GGVQSTASSK NASCYFDIEW RDRRITLRAS NGKFVTSKKN GQLAASVETA GDSELFLMKL INRPIIVFRG EHGFIG
• Sequence Length: 493

• Applicable Applications for FSCN recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Gln277~Gly396 (Accession # Q16658) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 4507115
• NCBI GeneID: 6624
• NCBI Accession #: NP_003079.1
• NCBI GenBank Nucleotide #: NM_003088.3
• UniProt Accession #: Q16658; Q96IC5; Q9BRF1; A6NI89; B2RE97
• Molecular Weight: 15.0kDa
• NCBI Official Full Name: fascin
• NCBI Official Synonym Full Names: fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus)
• NCBI Official Symbol: FSCN1
• NCBI Official Synonym Symbols: HSN; SNL; p55; FAN1
• NCBI Protein Information: fascin; 55 kDa actin-bundling protein; singed-like (fascin homolog, sea urchin)
• UniProt Protein Name: Fascin
• UniProt Gene Name: FSCN1
• UniProt Synonym Gene Names: FAN1; HSN; SNL
• UniProt Entry Name: FSCN1_HUMAN

NCBI Description

This gene encodes a member of the fascin family of actin-binding proteins. Fascin proteins organize F-actin into parallel bundles, and are required for the formation of actin-based cellular protrusions. The encoded protein plays a critical role in cell migration, motility, adhesion and cellular interactions. Expression of this gene is known to be regulated by several microRNAs, and overexpression of this gene may play a role in the metastasis of multiple types of cancer by increasing cell motility. Expression of this gene is also a marker for Reed-Sternberg cells in Hodgkin's lymphoma. A pseudogene of this gene is located on the long arm of chromosome 15. [provided by RefSeq, Sep 2011]

Uniprot Description

Fascin: Organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration. Associates with beta-catenin. Ubiquitous. Belongs to the fascin family.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis; Actin-binding

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: cytoplasm; stress fiber; cell junction; cytosol; filopodium; actin cytoskeleton

Molecular Function: actin filament binding; protein binding, bridging; protein binding; drug binding; actin binding

Biological Process: actin filament bundle formation; cell proliferation; cell migration; cell motility involved in cell locomotion; actin cytoskeleton organization and biogenesis