SDS-PAGE
FRS3 recombinant protein
FRS3 recombinant protein
Gene Names
FRS3; SNT2; FRS2B; SNT-2; FRS2beta; FRS2-beta
Applications
Western Blot
Synonyms
FRS3; FRS3 recombinant protein; SNT2; FRS2B; FRS2beta; MGC17167
Host
Sf9 insect cells
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Sequence Length
2198
Applicable Applications for FRS3 recombinant protein
Kinase Assay, Western Blot (WB)
Type
Recombinant Fusion Protein
Species
Human
Tag Information
GST tag
Expression System
Sf9 insect cells using baculovirus
Source Note
Recombinant full-length human FRS3 was expressed by baculovirus in Sf9 insect cells
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
SDS-PAGE
SDS-PAGE
Related Product Information for FRS3 recombinant protein
Recombinant full-length human FRS3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: FRS3 or fibroblast growth factor receptor substrate 3 is a peripheral plasma membrane protein that is a substrate for the fibroblast growth factor receptor. During FGF or NGF stimulation, FRS3 becomes tyrosine phosphorylated and then serves as a platform for the recruitment of multiple signaling proteins for activation of the Ras-MAP kinase signaling cascade (1). FRS3 is mainly expressed in fibroblast and myoblast cell lines and undergo robust tyrosine phosphorylation in response to several mitogenic ligands. Through interaction with Rho family of small GTPases, FRS3 has been implicated in the reorganization of the actin cytoskeleton and subsequent morphological changes in various cells (2).
Scientific Background: FRS3 or fibroblast growth factor receptor substrate 3 is a peripheral plasma membrane protein that is a substrate for the fibroblast growth factor receptor. During FGF or NGF stimulation, FRS3 becomes tyrosine phosphorylated and then serves as a platform for the recruitment of multiple signaling proteins for activation of the Ras-MAP kinase signaling cascade (1). FRS3 is mainly expressed in fibroblast and myoblast cell lines and undergo robust tyrosine phosphorylation in response to several mitogenic ligands. Through interaction with Rho family of small GTPases, FRS3 has been implicated in the reorganization of the actin cytoskeleton and subsequent morphological changes in various cells (2).
Product Categories/Family for FRS3 recombinant protein
References
1.Huang L, et al: SNT-2 interacts with ERK2 and negatively regulates ERK2 signaling in response to EGF stimulation. Biochem Biophys Res Commun. 2004 Nov 19;324(3):1011-7.
2.Xu H, et al: Novel recognition motif on fibroblast growth factor receptor mediates direct association and activation of SNT adapter proteins. J. Biol. Chem. 273: 17987-17990, 1998.
2.Xu H, et al: Novel recognition motif on fibroblast growth factor receptor mediates direct association and activation of SNT adapter proteins. J. Biol. Chem. 273: 17987-17990, 1998.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~87 kDa
NCBI Official Full Name
Homo sapiens fibroblast growth factor receptor substrate 3 (FRS3), mRNA
NCBI Official Synonym Full Names
fibroblast growth factor receptor substrate 3
NCBI Official Symbol
FRS3
NCBI Official Synonym Symbols
SNT2; FRS2B; SNT-2; FRS2beta; FRS2-beta
NCBI Protein Information
fibroblast growth factor receptor substrate 3; FGFR substrate 3; FGFR-signaling adaptor SNT2; suc1-associated neurotrophic factor target 2 (FGFR signalling adaptor)
UniProt Protein Name
Fibroblast growth factor receptor substrate 3
Protein Family
UniProt Gene Name
FRS3
UniProt Synonym Gene Names
FGFR substrate 3; SNT-2
UniProt Entry Name
FRS3_HUMAN
NCBI Description
This gene encodes a substrate for the fibroblast growth factor receptor. The encoded protein is found in the peripheral plasma membrane and links fibroblast growth factor receptor stimulation to activators of Ras. The encoded protein down-regulates extracellular regulated kinase 2 through direct binding. [provided by RefSeq, Jul 2013]
Uniprot Description
Function: Adapter protein that links FGF and NGF receptors to downstream signaling pathways. Involved in the activation of MAP kinases. Down-regulates ERK2 signaling by interfering with the phosphorylation and nuclear translocation of ERK2. Ref.6
Subunit structure: Binds NTRK1
By similarity. Binds FGFR1, NGFR, GRB2, PTPN11 and ERK2.
Subcellular location: Membrane; Lipid-anchor.
Post-translational modification: Phosphorylated by ULK2 in vitro
By similarity. Phosphorylated on tyrosine residues upon stimulation by BFGF or NGFB. Ref.1 Ref.6
Sequence similarities: Contains 1 IRS-type PTB domain.
Sequence caution: The sequence CAI13184.2 differs from that shown. Reason: Erroneous gene model prediction.
Research Articles on FRS3
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Product Notes
The FRS3 frs3 (Catalog #AAA515408) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is GST tag!!Expression System||Sf9 insect cells using baculovirus!!Source Note||Recombinant full-length human FRS3 was expressed by baculovirus in Sf9 insect cells. AAA Biotech's FRS3 can be used in a range of immunoassay formats including, but not limited to, Kinase Assay, Western Blot (WB). Researchers should empirically determine the suitability of the FRS3 frs3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FRS3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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