Forkhead Box Protein O1 (FOXO1) Recombinant Protein | FOXO1 recombinant protein

Recombinant Forkhead Box Protein O1 (FOXO1)

Cat. Num. AAA2018212

• Gene Names: Foxo1; Afxh; FKHR; Fkhr1; Foxo1a; AI876417
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Forkhead Box Protein O1 (FOXO1); Recombinant Forkhead Box Protein O1 (FOXO1); FOXO1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- RVQNEGTGK SSWWMLNPEG GKSGKSPRRR AASMDNNSKF AKSRGRAAKK KASLQSGQEG PGDSPGSQFS KWPASPGSHS NDDFDNWSTF RPRTSSNAST ISGRLSPIMT EQDDLGDGDV HSLVYPPSAA KMASTLPSLS EISNPENMEN LLDNLNLLSS PTSLTVSTQS SPGSMMQQTP CYSFAPPNTS LNSPSPNYSK YTYGQSSMSP LPQMPMQTLQ DSKSSYGGLN QYNCAPGLLK ELLTSDSPP
• Sequence Length: 652

• Applicable Applications for FOXO1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Arg222~Pro469 (Accession # Q9R1E0) with two N-terminal Tags, His-tag and T7-tag
• Subcellular Location: Cytoplasm. Nucleus
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 8.8
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 34328255
• NCBI GeneID: 56458
• NCBI Accession #: NP_062713.2
• NCBI GenBank Nucleotide #: NM_019739.3
• UniProt Accession #: Q9R1E0; Q9JJW4
• Molecular Weight: 30.4kDa
• NCBI Official Full Name: forkhead box protein O1
• NCBI Official Synonym Full Names: forkhead box O1
• NCBI Official Symbol: Foxo1
• NCBI Official Synonym Symbols: Afxh; FKHR; Fkhr1; Foxo1a; AI876417
• NCBI Protein Information: forkhead box protein O1; forkhead box O1a; forkhead protein 1; forkhead box protein O1A; forkhead in rhabdomyosarcoma
• UniProt Protein Name: Forkhead box protein O1
• Protein Family: Forkhead box protein
• UniProt Gene Name: Foxo1
• UniProt Synonym Gene Names: Fkhr; Foxo1a
• UniProt Entry Name: FOXO1_MOUSE

Uniprot Description

FOXO1A: a transcription factor of the forkhead family. A central regulator of metabolism in several cell types. May play an important role in myogenic growth and differentiation, and in the regulation of lipolysis in adipocytes by controlling the expression of adipose triglyceride lipase (ATGL), the rate-limiting lipolytic enzyme. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. Contains 1 fork-head domain.

Protein type: DNA-binding; Nuclear receptor co-regulator; Transcription factor; Oncoprotein

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function: protein binding; DNA binding; protein phosphatase 2A binding; sequence-specific DNA binding; ubiquitin protein ligase binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; blood vessel development; transcription, DNA-dependent; positive regulation of apoptosis; apoptosis; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; negative regulation of fat cell differentiation; negative regulation of stress-activated MAPK cascade; glucose homeostasis; cellular response to starvation; regulation of cell proliferation; regulation of transcription from RNA polymerase II promoter; positive regulation of gluconeogenesis; cellular response to insulin stimulus; regulation of transcription, DNA-dependent; positive regulation of protein catabolic process; insulin receptor signaling pathway; autophagy; positive regulation of transcription from RNA polymerase II promoter; cell differentiation; response to DNA damage stimulus; positive regulation of autophagy; negative regulation of apoptosis