Irisin/FNDC5 Recombinant Protein | FNDC5 recombinant protein

Recombinant Human/Mouse/Rat Irisin/FNDC5 (N-6His)

Cat. Num. AAA2569336

• Gene Names: FNDC5; FRCP2; irisin
• Purity: >95% as determined by reducing SDS-PAGE
• Synonyms: Irisin/FNDC5; Recombinant Human/Mouse/Rat Irisin/FNDC5 (N-6His); Fibronectin type III domain-containing protein 5; Fibronectin type III repeat-containing protein 2; Irisin; FNDC5; FNDC5 recombinant protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE
• Form/Format: Lyophilized from a 0.2um filtered solution of PBS, pH7.4.
• Sequence: Asp32-Glu143

• Species: Human, Mouse, Rat
• Tag: N-6His
• Endotoxin: <1.0EU per ug as determined by the LAL method.
• Protein Construction: Recombinant Human/Mouse/Rat Fibronectin Type III Domain-containing Protein 5 is produced by our Mammalian expression system and the target gene encoding Asp32-Glu143 is expressed with a 6His tag at the N-terminus.
• Preparation and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.; This product is provided as lyophilized powder which is shipped with ice packs.

Related Product Information for FNDC5 recombinant protein

Fibronectin type III domain-containing protein 5, the precursor of irisin, is a protein that is encoded by the FNDC5 gene.Human Irisin is synthesized as a 212 amino acid (aa) precursor encoding a type 1 transmembrane protein with a 121 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 39 aa cytoplasmic domain. The ECD of Irisin contains a fibronectin type III domain and multiple glycosylation sites. The ECD is proteolytically cleaved to release the 112 aa soluble Irisin hormone into circulation.Mature human, mouse share 100% sequence identity.Irisin induces expression of peroxisome proliferatoractivated receptor gamma coactivator 1alpha (PGC1alpha) and uncoupling protein1(UCP1), mitochondrialassociated metabolic proteins. Irisin induces the transition of white adipose tissue into more metabolically active beige adipose tissue.Irisin also regulates neuronal cell differentiation and neurite outgrowth in the brain and is involved in the differentiation of osteoblasts.

NCBI and Uniprot Product Information

• NCBI GI #: 511093995
• NCBI GeneID: 252995
• NCBI Accession #: NP_001165411.2
• NCBI GenBank Nucleotide #: NM_001171940.1
• UniProt Accession #: Q8NAU1; Q6P6D9; Q7Z676; A6NMC9; D3DPQ6
• Molecular Weight: 15,527 Da
• NCBI Official Full Name: fibronectin type III domain-containing protein 5 isoform 3 preproprotein
• NCBI Official Synonym Full Names: fibronectin type III domain containing 5
• NCBI Official Symbol: FNDC5
• NCBI Official Synonym Symbols: FRCP2; irisin
• NCBI Protein Information: fibronectin type III domain-containing protein 5; fibronectin type III repeat-containing protein 2
• UniProt Protein Name: Fibronectin type III domain-containing protein 5
• Protein Family: Fibronectin type III domain-containing protein
• UniProt Gene Name: FNDC5
• UniProt Synonym Gene Names: FRCP2
• UniProt Entry Name: FNDC5_HUMAN

NCBI Description

This gene encodes a secreted protein that is released from muscle cells during exercise. The encoded protein may participate in the development of brown fat. Translation of the precursor protein initiates at a non-AUG start codon at a position that is conserved as an AUG start codon in other organisms. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

Uniprot Description

FNDC5: Irisin: mediates beneficial effects of muscular exercise. Induces browning of white adipose tissue by stimulating UCP1 expression, at least in part, via the nuclear receptor PPARA. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p35.1

Cellular Component: peroxisomal membrane; endoplasmic reticulum; plasma membrane; integral to membrane; extracellular region

Molecular Function: hormone activity

Biological Process: response to muscle activity