Typical Testing Data/Standard Curve (for reference only)
Dog Fibronectin ELISA Kit | FN1 elisa kit
Dog Fibronectin ELISA Kit
Gene Names
FN1; FN
Reactivity
Dog
Synonyms
Fibronectin; Dog Fibronectin ELISA Kit; Canis lupus familiaris; FN; FN1; FN1 elisa kit
Reactivity
Dog
Specificity
This assay recognizes recombinant and natural dog FN. No significant cross-reactivity or interference was observed.
Samples
Serum, plasma, tissue homogenates and cell culture supernates and other biological fluids
Detection Range
1.S6ng/mL -1 00 ng/mL
Preparation and Storage
Store at 4 degree C
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for FN1 elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of dog fibronectin,FN concentrations in serum, plasma, tissue homogenates and cell culture supernates and other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FN. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for FN and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain FN, biotin-conjugated antibody and enzymeconjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of FN in the samples is then determined by comparing the 0.0. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FN. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for FN and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain FN, biotin-conjugated antibody and enzymeconjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of FN in the samples is then determined by comparing the 0.0. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
29,486 Da
NCBI Official Full Name
Fibronectin
NCBI Official Symbol
FN1
NCBI Official Synonym Symbols
FN
NCBI Protein Information
fibronectin
UniProt Protein Name
Fibronectin
Protein Family
UniProt Gene Name
FN1
UniProt Synonym Gene Names
FN
Uniprot Description
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts ().
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Product Notes
The Dog FN1 fn1 (Catalog #AAA9424962) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9424962 ELISA Kit recognizes Dog FN1. It is sometimes possible for the material contained within the vial of "Fibronectin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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