Fragile X Mental Retardation 1 Recombinant Protein | FMR1 recombinant protein

Recombinant Fragile X Mental Retardation 1 (FMR1)

Cat. Num. AAA2031779

• Gene Names: FMR1; POF; FMRP; POF1; FRAXA
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >90%
• Synonyms: Fragile X Mental Retardation 1; Recombinant Fragile X Mental Retardation 1 (FMR1); FMR1 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >90%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MEELVVEVRG SNGAFYKAFV KDVHEDSITV AFENNWQPDR QIPFHDVRFPPPVGYNKDIN ESDEVEVYSR ANEKEPCCWW LAKVRMIKGE FYVIEYAACDATYNEIVTIE RLRSVNPNKP ATKDTFHKIK LDVPEDLRQM CAKEAAHKDFKKAVGAFSVT YDPENYQLVI LSINEVTSKR AHMLIDMHFR SLRTKLSLIMRNEEASKQLE SSRQLASRFH EQFIVREDLM GLAIGTHGAN IQQARKVPGVTAIDLDEDTC TFHIYGEDQD AVKKARSFLE FAEDVIQVPR NLVGKVIGKNGKLIQEIVDK SGVV
• Sequence Length: 554

• Applicable Applications for FMR1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 39.6kDa
• Accurate Molecular Mass (KD): 40kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and S-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Met1~Val314
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 582054406
• NCBI GeneID: 2332
• Molecular Weight: 68,966 Da
• NCBI Official Full Name: fragile X mental retardation 1
• NCBI Official Synonym Full Names: fragile X mental retardation 1
• NCBI Official Symbol: FMR1
• NCBI Official Synonym Symbols: POF; FMRP; POF1; FRAXA
• NCBI Protein Information: fragile X mental retardation protein 1
• UniProt Protein Name: Fragile X mental retardation protein 1
• Protein Family: Fragile X mental retardation 1 neighbor protein
• UniProt Gene Name: FMR1
• UniProt Synonym Gene Names: FMRP; Protein FMR-1
• UniProt Entry Name: FMR1_HUMAN

NCBI Description

The protein encoded by this gene binds RNA and is associated with polysomes. The encoded protein may be involved in mRNA trafficking from the nucleus to the cytoplasm. A trinucleotide repeat (CGG) in the 5' UTR is normally found at 6-53 copies, but an expansion to 55-230 repeats is the cause of fragile X syndrome. Expansion of the trinucleotide repeat may also cause one form of premature ovarian failure (POF1). Multiple alternatively spliced transcript variants that encode different protein isoforms and which are located in different cellular locations have been described for this gene. [provided by RefSeq, May 2010]

Uniprot Description

FMR1: Translation repressor. Component of the CYFIP1-EIF4E- FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates translation repression. RNA- binding protein that plays a role in intracellular RNA transport and in the regulation of translation of target mRNAs. Associated with polysomes. May play a role in the transport of mRNA from the nucleus to the cytoplasm. Binds strongly to poly(G), binds moderately to poly(U) but shows very little binding to poly(A) or poly(C). Component of the CYFIP1-EIF4E-FMR1 complex which is composed of CYFIP, EIF4E and FMR1. Interacts with CYFIP1 and CYFIP2. The interaction with brain cytoplasmic RNA 1 (BC1) increases binding affinity for the CYFIP1-EIF4E complex in the brain. Homooligomer. Found in a RNP granule complex with IGF2BP1. Directly interacts with SMN and TDRD3. Interacts with the SMN core complex that contains SMN1, GEMIN2/SIP1, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8 and STRAP/UNRIP. Interacts with FXR1, FXR2, IGF2BP1, NUFIP1, NUFIP2, MCRS1 and RANBP9. Highest levels found in neurons, brain, testis, placenta and lymphocytes. Also expressed in epithelial tissues and at very low levels in glial cells. Belongs to the FMR1 family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus; RNA-binding; Translation

Chromosomal Location of Human Ortholog: Xq27.3

Cellular Component: nucleoplasm; polysomal ribosome; mRNA cap complex; microtubule; membrane; stress granule; cytoplasm; nucleolus; dendritic spine; synapse; dendritic shaft

Molecular Function: mRNA binding; protein binding; RNA binding; microtubule binding

Biological Process: negative regulation of translational initiation; mRNA transport; central nervous system development; negative regulation of translation

Disease: Fragile X Mental Retardation Syndrome; Fragile X Tremor/ataxia Syndrome; Premature Ovarian Failure 1