Flavin Containing Monooxygenase 2 Recombinant Protein | FMO2 recombinant protein

Recombinant Flavin Containing Monooxygenase 2, Non Functional (FMO2)

Cat. Num. AAA2033184

• Gene Names: FMO2; FMO1B1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Flavin Containing Monooxygenase 2; Recombinant Flavin Containing Monooxygenase 2; Non Functional (FMO2); FMO1B1; Dimethylaniline oxidase 2; Pulmonary flavin-containing monooxygenase 2; Dimethylaniline monooxygenase [N-oxide-forming] 2; FMO2 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Sequence: RPDFA SSGQWDVYVQ SNGKEQRAVF DAVMVCSGHH IQPHLPLKSF PGIERFQGQY FHSRQYKHPV GYEGKRILVV GIGNSAADIA SELSKRAAQV FVSTRHGSWV LSRISEDGYP WDMVFHTRFS SMLRNVLPRT VVKWMMERQM NRWFNHENYG LVPQNKYLMKEPVLNDDLPS RLLYGAIKVK TRVKELTETA VVFEDGTVEE DVDV
• Sequence Length: 471

• Applicable Applications for FMO2 recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 27.8kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Arg116~Val324
• Organism Species: Rat
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 119611295
• NCBI GeneID: 2327
• Molecular Weight: 53,644 Da
• NCBI Official Full Name: flavin containing monooxygenase 2
• NCBI Official Synonym Full Names: flavin containing monooxygenase 2
• NCBI Official Symbol: FMO2
• NCBI Official Synonym Symbols: FMO1B1
• NCBI Protein Information: dimethylaniline monooxygenase [N-oxide-forming] 2
• UniProt Protein Name: Dimethylaniline monooxygenase [N-oxide-forming] 2
• Protein Family: Dimethylaniline monooxygenase
• UniProt Gene Name: FMO2
• UniProt Synonym Gene Names: FMO 2
• UniProt Entry Name: FMO2_HUMAN

NCBI Description

This gene encodes a flavin-containing monooxygenase family member. It is an NADPH-dependent enzyme that catalyzes the N-oxidation of some primary alkylamines through an N-hydroxylamine intermediate. However, some human populations contain an allele (FMO2*2A) with a premature stop codon, resulting in a protein that is C-terminally-truncated, has no catalytic activity, and is likely degraded rapidly. This gene is found in a cluster with other related family members on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2014]

Uniprot Description

Catalyzes the N-oxidation of certain primary alkylamines to their oximes via an N-hydroxylamine intermediate. Inactive toward certain tertiary amines, such as imipramine or chloropromazine. Can catalyze the S-oxidation of methimazole. The truncated form is catalytically inactive.