Serum Amyloid P Component (SAP) Recombinant Protein | SAP recombinant protein

Recombinant Serum Amyloid P Component (SAP)

Cat. Num. AAA2009614

• Gene Names: Apcs; Sap
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Serum Amyloid P Component (SAP); Recombinant Serum Amyloid P Component (SAP); SAP recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGS- TDLNQKVFV FPRESETDYV KLIPWLEKPL QNFTLCFRAY SDLSRSQSLF SYSVNSRDNE LLIYKDKVGQ YSLYIGNSKV TVRGLEEFPS PIHFCTSWES SSGIAEFWVN GKPWVKKGLQ KGYTVKSSPS IVLGQEQDTY GGGFDKTQSF VGEIADLYMW DSVLTPENIH SVDRGFPPNP NILDWRALNY EINGYVVIKP RM
• Sequence Length: 228

• Applicable Applications for SAP recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Thr22~Met222 (Accession # P23680) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 148747488
• NCBI GeneID: 29339
• NCBI Accession #: NP_058866.2
• NCBI GenBank Nucleotide #: NM_017170.2
• UniProt Accession #: P23680; Q63539
• Molecular Weight: 24.3kDa
• NCBI Official Full Name: serum amyloid P-component
• NCBI Official Synonym Full Names: amyloid P component, serum
• NCBI Official Symbol: Apcs
• NCBI Official Synonym Symbols: Sap
• NCBI Protein Information: serum amyloid P-component
• UniProt Protein Name: Serum amyloid P-component
• Protein Family: S-layer protein
• UniProt Gene Name: Apcs
• UniProt Synonym Gene Names: Ptx2; Sap; SAP
• UniProt Entry Name: SAMP_RAT

NCBI Description

a component of amyloid P [RGD, Feb 2006]

Uniprot Description

Cofactor: Binds 2 calcium ions per subunit

By similarity.

Subunit structure: Homopentamer. Pentaxin (or pentraxin) have a discoid arrangement of 5 non-covalently bound subunits.

Subcellular location: Secreted.

Involvement in disease: SAP is a precursor of amyloid component P which is found in basement membrane and associated with amyloid deposits.

Sequence similarities: Belongs to the pentaxin family.Contains 1 pentaxin domain.