Bovine CX3C-chemokine/Fractalkine ELISA Kit | FKN elisa kit

Bovine CX3C-chemokine/Fractalkine ELISA Kit

Cat. Num. AAA737492

• Gene Names: CX3CL1; NTN; NTT; CXC3; CXC3C; SCYD1; ABCD-3; C3Xkine; fractalkine; neurotactin
• Reactivity: Bovine
• Synonyms: CX3C-chemokine/Fractalkine; Bovine CX3C-chemokine/Fractalkine ELISA Kit; FKN elisa kit

• Reactivity: Bovine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This FKN/CX3CL1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine FKN/CX3CL1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FKN elisa kit

Principle of the Assay: FKN/CX3CL1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for FKN/CX3CL1. Standards or samples are then added to the microtiter plate wells and FKN/CX3CL1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of FKN/CX3CL1 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for FKN/CX3CL1 are added to each well to "sandwich" the FKN/CX3CL1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain FKN/CX3CL1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FKN/CX3CL1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for FKN elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 4506857
• NCBI GeneID: 6376
• NCBI Accession #: NP_002987
• NCBI GenBank Nucleotide #: NM_002996.3
• UniProt Accession #: P78423; O00672
• Molecular Weight: 42,203 Da
• NCBI Official Full Name: fractalkine
• NCBI Official Synonym Full Names: chemokine (C-X3-C motif) ligand 1
• NCBI Official Symbol: CX3CL1
• NCBI Official Synonym Symbols: NTN; NTT; CXC3; CXC3C; SCYD1; ABCD-3; C3Xkine; fractalkine; neurotactin
• NCBI Protein Information: fractalkine; C-X3-C motif chemokine 1; small-inducible cytokine D1; CX3C membrane-anchored chemokine; small inducible cytokine subfamily D (Cys-X3-Cys), member-1; small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin)
• UniProt Protein Name: Fractalkine
• UniProt Gene Name: CX3CL1
• UniProt Synonym Gene Names: FKN; NTT; SCYD1
• UniProt Entry Name: X3CL1_HUMAN

Uniprot Description

CX3CL1: The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1. Belongs to the intercrine delta family.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 16q13

Cellular Component: extracellular space; cell surface; extracellular region; plasma membrane; integral to membrane

Molecular Function: protein binding; chemokine activity; receptor binding

Biological Process: neutrophil chemotaxis; positive regulation of transforming growth factor-beta1 production; leukocyte chemotaxis; cytokine and chemokine mediated signaling pathway; defense response; angiogenesis involved in wound healing; chemotaxis; macrophage chemotaxis; leukocyte adhesive activation; positive regulation of angiogenesis; positive regulation of calcium-independent cell-cell adhesion; immune response; cell adhesion; lymphocyte chemotaxis; positive regulation of inflammatory response