Fish High density lipoprotein (HDL) ELISA Kit | HDL elisa kit
Fish High density lipoprotein (HDL) ELISA Kit
Reactivity
Fish
Synonyms
High density lipoprotein (HDL); Fish High density lipoprotein (HDL) ELISA Kit; HDL elisa kit
Reactivity
Fish
Specificity
This assay has high sensitivity and excellent specificity for detection of Fish HDL. No significant cross-reactivity or interference between Fish HDL and analogues was observed.
Sequence Length
17
Samples
Serum, plasma and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
3.12 ug/mL - 200 ug/mL
Sensitivity
1.56 ug/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for HDL elisa kit
Intended Uses: For the quantitative detection of Fish High density lipoprotein (HDL) concentration in serum, plasma and other biological fluids.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate HDL in Fish serum, plasma. An antibody specific for HDL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HDL present is bound by the immobilized antibody. After removing any unbound substances, a biotinconjugated antibody specific for HDL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HDL bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background/Introduction: High-density lipoproteins (HDL) form a class of lipoproteins, varying somewhat in their size (8-11 nm in diameter), that carry cholesterol from the bodys tissues to the liver. About thirty percent of blood cholesterol is carried by HDL. HDL is the smallest of the lipoprotein particles. They are the densest because they contain the highest proportion of protein. It is hypothesised that HDL can remove cholesterol from atheroma within arteries and transport it back to the liver for excretion or re-utilization-which is the main reason why HDL-bound cholesterol is sometimes called "good cholesterol", or HDL-C. A high level of HDL-C seems to protect against cardiovascular diseases, and low HDL cholesterol levels (less than 40 mg/dL) increase the risk for heart disease. When measuring cholesterol, any contained in HDL particles is considered as protection to the bodys cardiovascular health, in contrast to "bad" LDL cholesterol.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate HDL in Fish serum, plasma. An antibody specific for HDL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HDL present is bound by the immobilized antibody. After removing any unbound substances, a biotinconjugated antibody specific for HDL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HDL bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background/Introduction: High-density lipoproteins (HDL) form a class of lipoproteins, varying somewhat in their size (8-11 nm in diameter), that carry cholesterol from the bodys tissues to the liver. About thirty percent of blood cholesterol is carried by HDL. HDL is the smallest of the lipoprotein particles. They are the densest because they contain the highest proportion of protein. It is hypothesised that HDL can remove cholesterol from atheroma within arteries and transport it back to the liver for excretion or re-utilization-which is the main reason why HDL-bound cholesterol is sometimes called "good cholesterol", or HDL-C. A high level of HDL-C seems to protect against cardiovascular diseases, and low HDL cholesterol levels (less than 40 mg/dL) increase the risk for heart disease. When measuring cholesterol, any contained in HDL particles is considered as protection to the bodys cardiovascular health, in contrast to "bad" LDL cholesterol.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
high density lipoprotein, HDL {N-terminal}
Protein Family
Similar Products
Product Notes
The Fish HDL (Catalog #AAA2800392) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2800392 ELISA Kit recognizes Fish HDL. It is sometimes possible for the material contained within the vial of "High density lipoprotein (HDL), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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