Mouse Interleukin 1 Delta ELISA Kit | FIL1d elisa kit

Mouse Interleukin 1 Delta ELISA Kit

Cat. Num. AAA764796

• Reactivity: Mouse
• Synonyms: Interleukin 1 Delta; Mouse Interleukin 1 Delta ELISA Kit; FIL1d; FIL1d elisa kit

• Reactivity: Mouse
• Sequence Length: 155

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 31.25-2000pg/ml
• Sensitivity: 18.75pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for FIL1d elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse FIL1d antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse FIL1d antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse FIL1d amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse FIL1d can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 9651789
• Molecular Weight: 16,962 Da
• NCBI Official Full Name: interleukin-1 delta
• UniProt Protein Name: Interleukin-36 receptor antagonist protein
• UniProt Gene Name: IL36RN
• UniProt Synonym Gene Names: FIL1D; IL1F5; IL1HY1; IL1L1; IL1RP3; IL-1RP3; IL-1HY1; IL-1 delta; IL-1F5; IL-1ra homolog 1; IL-1L1
• UniProt Entry Name: I36RA_HUMAN

Uniprot Description

IL1F5: Is a highly and a specific antagonist of the IL-1 receptor-related protein 2-mediated response to interleukin 1 family member 9 (IL1F9). Could constitute part of an independent signaling system analogous to interleukin-1 alpha (IL-1A), beta (IL-1B) receptor agonist and interleukin-1 receptor type I (IL- 1R1), that is present in epithelial barriers and takes part in local inflammatory response. Defects in IL36RN are the cause of psoriasis generalized pustular (PSORP). PSORP is a life-threatening disease defined by repeated flares of sudden onset consisting of diffuse erythematous skin eruption characterized by rapid coverage with pustules, high-grade fever, asthenia, marked leukocytosis, and elevated serum levels of C-reactive protein. Belongs to the IL-1 family.

Protein type: Cytokine

Chromosomal Location of Human Ortholog: 2q14

Cellular Component: extracellular space

Molecular Function: cytokine activity; interleukin-1 receptor antagonist activity; interleukin-1 receptor binding; protein binding

Biological Process: antifungal humoral response; cytokine and chemokine mediated signaling pathway; inflammatory response to antigenic stimulus; innate immune response; negative regulation of cytokine and chemokine mediated signaling pathway; negative regulation of interleukin-17 production; negative regulation of interleukin-6 production

Disease: Psoriasis 14, Pustular