Polycomb group protein FERTILIZATION-INDEPENDENT ENDOSPERM (FIE) Recombinant Protein | FIE recombinant protein

Recombinant Arabidopsis thaliana Polycomb group protein FERTILIZATION-INDEPENDENT ENDOSPERM (FIE)

Cat. Num. AAA1304468

• Gene Names: FIE; FERTILIZATION-INDEPENDENT ENDOSPERM; FERTILIZATION-INDEPENDENT ENDOSPERM 1; FIE1; FIS3
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Polycomb group protein FERTILIZATION-INDEPENDENT ENDOSPERM (FIE); Recombinant Arabidopsis thaliana Polycomb group protein FERTILIZATION-INDEPENDENT ENDOSPERM (FIE); FIE recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-369, Full length protein
• Sequence: MSKITLGNESIVGSLTPSNKKSYKVTNRIQEGKKPLYAVVFNFLDARFFDVFVTAGGNRITLYNCLGDGAISALQSYADEDKEESFYTVSWACGVNGNPYVAAGGVKGIIRVIDVNSETIHKSLVGHGDSVNEIRTQPLKPQLVITASKDESVRLWNVETGICILIFAGAGGHRYEVLSVDFHPSDIYRFASCGMDTTIKIWSMKEFWTYVEKSFTWTDDPSKFPTKFVQFPVFTASIHTNYVDCNRWFGDFILSKSVDNEILLWEPQLKENSPGEGASDVLLRYPVPMCDIWFIKFSCDLHLSSVAIGNQEGKVYVWDLKSCPPVLITKLSHNQSKSVIRQTAMSVDGSTILACCEDGTIWRWDVITK
• Sequence Length: 369

• Species: Arabidopsis thaliana (Mouse-ear cress)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 15232345
• NCBI GeneID: 821622
• NCBI Accession #: NP_188710.1
• NCBI GenBank Nucleotide #: NM_112965.1
• UniProt Accession #: Q9LT47; Q9XF44
• Molecular Weight: 41,257 Da
• NCBI Official Full Name: Transducin/WD40 repeat-like superfamily protein
• NCBI Official Symbol: FIE
• NCBI Official Synonym Symbols: FERTILIZATION-INDEPENDENT ENDOSPERM; FERTILIZATION-INDEPENDENT ENDOSPERM 1; FIE1; FIS3
• NCBI Protein Information: Transducin/WD40 repeat-like superfamily protein
• UniProt Protein Name: Polycomb group protein FERTILIZATION-INDEPENDENT ENDOSPERM
• Protein Family: Polycomb group protein
• UniProt Gene Name: FIE
• UniProt Synonym Gene Names: FIS3

NCBI Description

Encodes a protein similar to the transcriptional regular of the animal Polycomb group and is involved in regulation of establishment of anterior-posterior polar axis in the endosperm and repression of flowering during vegetative phase. Mutation leads endosperm to develop in the absence of fertilization and flowers to form in seedlings and non-reproductive organs. Also exhibits maternal effect gametophytic lethal phenotype, which is suppressed by hypomethylation. Forms part of a large protein complex that can include VRN2 (VERNALIZATION 2), VIN3 (VERNALIZATION INSENSITIVE 3) and polycomb group proteins FERTILIZATION INDEPENDENT ENDOSPERM (FIE), CURLY LEAF (CLF) and SWINGER (SWN or EZA1). The complex has a role in establishing FLC (FLOWERING LOCUS C) repression during vernalization. In the ovule, the FIE transcript levels increase transiently just after fertilization.

Uniprot Description

Polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. They probably act via the methylation of histones, rendering chromatin heritably changed in its expressibility. Required to prevent the proliferation of the central cell by repressing unknown target genes before fertilization. Probably also involved in floral repression mechanism established during early plant development. Regulates the anteroposterior organization of the endosperm. Interacts with the promoter and represses the transcription of genes such as PHE1, that are paternally active and maternally silenced.