Fetuin-A / AHSG Recombinant Protein | AHSG recombinant protein

Recombinant Mouse Fetuin-A / AHSG Protein (His tag)

Cat. Num. AAA2547166

• Gene Names: Fgfr4; Fgfr-4
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: Fetuin-A / AHSG; Recombinant Mouse Fetuin-A / AHSG Protein (His tag); fetuin-A; Ahsg; AHSG recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 25mM Tris, 0.15mM NaCl, pH 7.5
• Sequence Length: 799

• Application Notes: The secreted recombinant mouse AHSG consists of 338 amino acids and has a predicted molecular mass of 36.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm AHSG is approximately 50 kDa due to glycosylation.
• Predicted N Terminal: Ala 19
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for AHSG recombinant protein

Background: Fetuin-A, also known as Alpha-2-HS-Glycoprotein (AHSG), belongs to the Fetuin family, is a plasma binding protein, and is more abundant in fetal than adult blood. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. Fetuins are carrier proteins like albumin. Fetuin-A forms soluble complexes with calcium and phosphate and thus is a carrier of otherwise insoluble calcium phosphate. Thus Fetuin-A is a potent inhibitor of pathological calcification. The circulating levels of fetuin-A, a well-described inhibitor of calcification, regulate the cell-dependent process of osteogenesis. The low circulating fetuin-A levels are associated with a greater prevalence and/or severity of Vascular calcification (VC) and increased risk for all-cause and cardiovascular mortality. However, high circulating fetuin-A levels appear to induce insulin resistance and, in non-dialyzed subjects with diabetic nephropathy, are directly related to VC burden. The emerging role of fetuin-A deficiency as a risk factor in dialysis patients was documented in cross-sectional studies demonstrating a significant correlation with all-cause and cardiovascular mortality. Additionally, Human fetuin-A is a negative acute phase protein involved in inflammatory diseases, thus being a potential physiological regulator of meprin activity. Fetuin-A is a broad-range protease inhibitor. Fetuin-A and cystatin C as endogenous proteolytic regulators of meprin activity broadens our understanding of the proteolytic network in plasma.

Description: A DNA sequence encoding the mouse AHSG (NP_032037.2) precursor (Met1-Ile 345) was expressed with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 112293260
• NCBI GeneID: 14186
• NCBI Accession #: NP_032037.2
• NCBI GenBank Nucleotide #: NM_008011.2
• UniProt Accession #: Q03142; Q27Q87; Q5J7D9; Q8C3V5; Q8CIB8
• Molecular Weight: 83,335 Da
• NCBI Official Full Name: fibroblast growth factor receptor 4
• NCBI Official Synonym Full Names: fibroblast growth factor receptor 4
• NCBI Official Symbol: Fgfr4
• NCBI Official Synonym Symbols: Fgfr-4
• NCBI Protein Information: fibroblast growth factor receptor 4
• UniProt Protein Name: Fibroblast growth factor receptor 4
• Protein Family: Alpha-2-HS-glycoprotein
• UniProt Gene Name: Fgfr4
• UniProt Synonym Gene Names: Fgfr-4; Mpk-11; FGFR-4

Uniprot Description

FGFR4: a tyrosine-kinase receptor that acts as cell-surface receptor for fibroblast growth factors and plays a role in the regulation of cell proliferation, differentiation and migration, and in regulation of lipid metabolism, bile acid biosynthesis, glucose uptake, vitamin D metabolism and phosphate homeostasis. Required for normal down-regulation of the expression of CYP7A1, the rate-limiting enzyme in bile acid synthesis, in response to FGF19. Phosphorylates PLCG1 and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of the MAP kinase and AKT signaling pathways. Promotes SRC-dependent phosphorylation of the matrix protease MMP14 and its lysosomal degradation. FGFR4 signaling is down-regulated by receptor internalization and degradation; MMP14 promotes internalization and degradation of FGFR4. Mutations that lead to constitutive kinase activation or impair normal FGFR4 inactivation lead to aberrant signaling. Two isoforms of the human protein are formed by alternative splicing. This description may include information from UniProtKB

Protein type: EC 2.7.10.1; FGFR family; Kinase, protein; Membrane protein, integral; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group

Chromosomal Location of Human Ortholog: 13 B1|13 29.8 cM

Cellular Component: cytoplasm; endoplasmic reticulum; Golgi apparatus; integral to plasma membrane; intercellular junction; nucleoplasm; plasma membrane; transport vesicle

Molecular Function: fibroblast growth factor binding; fibroblast growth factor receptor activity; heparin binding; protein binding

Biological Process: cell migration; fibroblast growth factor receptor signaling pathway; glucose homeostasis; induction of an organ; lung development; peptidyl-tyrosine phosphorylation; phosphate ion homeostasis; positive regulation of catalytic activity; positive regulation of cell proliferation; positive regulation of proteolysis; protein amino acid autophosphorylation; regulation of lipid metabolic process; regulation of phosphorus metabolic process; vacuolar phosphate transport