FGFR4 / CD334 recombinant protein

Recombinant Mouse FGFR4 / CD334 Protein (His tag)

Cat. Num. AAA2547108

• Gene Names: Fgfr4; Fgfr-4
• Purity: > 97 % as determined by SDS-PAGE
• Synonyms: FGFR4 / CD334; Recombinant Mouse FGFR4 / CD334 Protein (His tag); Fgfr-4; Fgfr4; Mpk-11; FGFR4 / CD334 recombinant protein

• Host: Human Cells
• Purity/Purification: > 97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 799

• Application Notes: The secreted recombinant mouse FGFR4 consists of 361 amino acids and has a predicted molecular mass of 40 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm FGFR4 is approximately 60-70 kDa due to glycosylation.
• Predicted N Terminal: Leu l7
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for FGFR4 / CD334 recombinant protein

Background: Fibroblast growth factor receptor 4 (FGFR4) also known as CD334 antigen or tyrosine kinase related to fibroblast growth factor receptor, is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of FGFR4/CD334 interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. FGFR4/CD334 preferentially binds acidic fibroblast growth factor and, although its specific function is unknown, it is overexpressed in gynecological tumor samples, suggesting a role in breast and ovarian tumorigenesis. FGFR4/CD334 signaling is down-regulated by receptor internalization and degradation; MMP14 promotes internalization and degradation of FGFR4/CD334. Mutations in FGFR4/CD334 lead to constitutive kinase activation or impair normal FGFR4 inactivation lead to aberrant signaling.

Description: A DNA sequence encoding the extracellular domain (Met 1-Asp 366) of mouse FGFR4 (NP_032037.2) precursor was expressed with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 112293260
• NCBI GeneID: 14186
• NCBI Accession #: NP_032037.2
• NCBI GenBank Nucleotide #: NM_008011.2
• UniProt Accession #: Q03142; Q27Q87; Q5J7D9; Q8C3V5; Q8CIB8
• Molecular Weight: 83,335 Da
• NCBI Official Full Name: fibroblast growth factor receptor 4
• NCBI Official Synonym Full Names: fibroblast growth factor receptor 4
• NCBI Official Symbol: Fgfr4
• NCBI Official Synonym Symbols: Fgfr-4
• NCBI Protein Information: fibroblast growth factor receptor 4
• UniProt Protein Name: Fibroblast growth factor receptor 4
• UniProt Gene Name: Fgfr4
• UniProt Synonym Gene Names: Fgfr-4; Mpk-11; FGFR-4

Uniprot Description

FGFR4: a tyrosine-kinase receptor that acts as cell-surface receptor for fibroblast growth factors and plays a role in the regulation of cell proliferation, differentiation and migration, and in regulation of lipid metabolism, bile acid biosynthesis, glucose uptake, vitamin D metabolism and phosphate homeostasis. Required for normal down-regulation of the expression of CYP7A1, the rate-limiting enzyme in bile acid synthesis, in response to FGF19. Phosphorylates PLCG1 and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of the MAP kinase and AKT signaling pathways. Promotes SRC-dependent phosphorylation of the matrix protease MMP14 and its lysosomal degradation. FGFR4 signaling is down-regulated by receptor internalization and degradation; MMP14 promotes internalization and degradation of FGFR4. Mutations that lead to constitutive kinase activation or impair normal FGFR4 inactivation lead to aberrant signaling. Two isoforms of the human protein are formed by alternative splicing. This description may include information from UniProtKB

Protein type: EC 2.7.10.1; FGFR family; Kinase, protein; Membrane protein, integral; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group

Chromosomal Location of Human Ortholog: 13 B1|13 29.8 cM

Cellular Component: cytoplasm; endoplasmic reticulum; Golgi apparatus; integral to plasma membrane; intercellular junction; nucleoplasm; plasma membrane; transport vesicle

Molecular Function: fibroblast growth factor binding; fibroblast growth factor receptor activity; heparin binding; protein binding

Biological Process: cell migration; fibroblast growth factor receptor signaling pathway; glucose homeostasis; induction of an organ; lung development; peptidyl-tyrosine phosphorylation; phosphate ion homeostasis; positive regulation of catalytic activity; positive regulation of cell proliferation; positive regulation of proteolysis; protein amino acid autophosphorylation; regulation of lipid metabolic process; regulation of phosphorus metabolic process; vacuolar phosphate transport