Typical Standard Curve/Testing Data
Bovine Fibroblast Growth Factor Binding Protein 2 ELISA Kit | FGFBP2 elisa kit
Bovine Fibroblast Growth Factor Binding Protein 2 ELISA Kit
Gene Names
FGFBP2; KSP37; HBP17RP
Reactivity
Bovine
Synonyms
Fibroblast Growth Factor Binding Protein 2; Bovine Fibroblast Growth Factor Binding Protein 2 ELISA Kit; FGFBP2 elisa kit
Reactivity
Bovine
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Standard Curve/Testing Data
Typical Standard Curve/Testing Data
Related Product Information for FGFBP2 elisa kit
Intended Uses: This FGFBP2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine FGFBP2. This ELISA kit for research use only!
Principle of the Assay: FGFBP2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-FGFBP2 antibody and an FGFBP2-HRP conjugate. The assay sample and buffer are incubated together with FGFBP2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FGFBP2 concentration since FGFBP2 from samples and FGFBP2-HRP conjugate compete for the anti-FGFBP2 antibody binding site. Since the number of sites is limited, as more sites are occupied by FGFBP2 from the sample, fewer sites are left to bind FGFBP2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FGFBP2 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: FGFBP2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-FGFBP2 antibody and an FGFBP2-HRP conjugate. The assay sample and buffer are incubated together with FGFBP2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FGFBP2 concentration since FGFBP2 from samples and FGFBP2-HRP conjugate compete for the anti-FGFBP2 antibody binding site. Since the number of sites is limited, as more sites are occupied by FGFBP2 from the sample, fewer sites are left to bind FGFBP2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FGFBP2 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for FGFBP2 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24,581 Da
NCBI Official Full Name
fibroblast growth factor-binding protein 2
NCBI Official Synonym Full Names
fibroblast growth factor binding protein 2
NCBI Official Symbol
FGFBP2
NCBI Official Synonym Symbols
KSP37; HBP17RP
NCBI Protein Information
fibroblast growth factor-binding protein 2; FGF-BP2; FGFBP-2; HBp17-RP; FGF-binding protein 2; HBp17-related protein; 37 kDa killer-specific secretory protein; killer-specific secretory protein of 37 kDa
UniProt Protein Name
Fibroblast growth factor-binding protein 2
Protein Family
UniProt Gene Name
FGFBP2
UniProt Synonym Gene Names
KSP37; UNQ425/PRO1065; FGF-BP2; FGF-binding protein 2; FGFBP-2; Ksp37; HBp17-RP
UniProt Entry Name
FGFP2_HUMAN
NCBI Description
This gene encodes a member of the fibroblast growth factor binding protein family. The encoded protein is a serum protein that is selectively secreted by cytotoxic lymphocytes and may be involved in cytotoxic lymphocyte-mediated immunity. An increase in the amount of gene product may be associated with atopic asthma and mild extrinsic asthma.[provided by RefSeq Staff, Oct 2008]
Uniprot Description
FGFBP2: a member of the fibroblast growth factor binding protein family. The encoded protein is a serum protein that is selectively secreted by cytotoxic lymphocytes and may be involved in cytotoxic lymphocyte-mediated immunity. An increase in the amount of gene product may be associated with atopic asthma and mild extrinsic asthma.[provided by RefSeq Staff, Oct 2008]
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 4p16
Cellular Component: extracellular space
Molecular Function: growth factor binding
Research Articles on FGFBP2
Similar Products
Product Notes
The Bovine FGFBP2 fgfbp2 (Catalog #AAA749884) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA749884 ELISA Kit recognizes Bovine FGFBP2. It is sometimes possible for the material contained within the vial of "Fibroblast Growth Factor Binding Protein 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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