Typical Testing Data/Standard Curve (for reference only)
Human Fibroblast Growth Factor 21 ELISA Kit | FGF 21 elisa kit
Human Fibroblast Growth Factor 21 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: FGF-21 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FGF-21 antibody and an FGF-21-HRP conjugate. The assay sample and buffer are incubated together with FGF-21-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FGF-21 concentration since FGF-21 from samples and FGF-21-HRP conjugate compete for the anti-FGF-21 antibody binding site. Since the number of sites is limited, as more sites are occupied by FGF-21 from the sample, fewer sites are left to bind FGF-21-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FGF-21 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. The function of this growth factor has not yet been determined. [provided by RefSeq, Jul 2008]
Uniprot Description
FGF21: Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. Belongs to the heparin-binding growth factors family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 19q13.33
Cellular Component: extracellular region
Molecular Function: protein binding; growth factor activity; fibroblast growth factor receptor binding
Biological Process: positive regulation of glucose import; cell-cell signaling; positive regulation of cell proliferation; positive regulation of low-density lipoprotein receptor biosynthetic process; signal transduction
Research Articles on FGF 21
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Product Notes
The Human FGF 21 fgf21 (Catalog #AAA754028) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA754028 ELISA Kit recognizes Human FGF 21. It is sometimes possible for the material contained within the vial of "Fibroblast Growth Factor 21, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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