Flap endonuclease 1 (Fen1) Recombinant Protein | Fen1 recombinant protein

Recombinant Mouse Flap endonuclease 1 (Fen1)

Cat. Num. AAA951230

• Gene Names: Fen1; FEN-1; AW538437
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Flap endonuclease 1 (Fen1); Recombinant Mouse Flap endonuclease 1 (Fen1); Fen1 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-378, Full length protein
• Sequence: MGIHGLAKLIADVAPSAIRENDIKSYFGRKVAIDASMSIYQFLIAVRQGGDVLQNEEGETTSLMGMFYRTIRMENGIKPVYVFDGKPPQLKSGELAKRSERRAEAEKQLQQAQEAGMEEEVEKFTKRLVKVTKQHNDECKHLLSLMGIPYLDAPSEAEASCAALAKAGKVYAAATEDMDCLTFGSPVLMRHLTASEAKKLPIQEFHLSRVLQELGLNQEQFVDLCILLGSDYCESIRGIGAKRAVDLIQKHKSIEEIVRRLDPSKYPVPENWLHKEAQQLFLEPEVVDPESVELKWSEPNEEELVKFMCGEKQFSEERIRSGVKRLSKSRQGSTQGRLDDFFKVTGSLSSAKRKEPEPKGPAKKKAKTGGAGKFRRGK
• Sequence Length: 378

• Species: Mouse
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Related Product Information for Fen1 recombinant protein

This protein removes 5 overhanging flaps in DNA repair and processes the 5 ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG
RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5 end of the flap that is necessary for both binding and cleavage by This protein. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.

NCBI and Uniprot Product Information

• NCBI GI #: 729476
• NCBI GeneID: 14156
• UniProt Accession #: P39749
• Molecular Weight: 42,315 Da
• NCBI Official Full Name: Flap endonuclease 1
• NCBI Official Synonym Full Names: flap structure specific endonuclease 1
• NCBI Official Symbol: Fen1
• NCBI Official Synonym Symbols: FEN-1; AW538437
• NCBI Protein Information: flap endonuclease 1
• UniProt Protein Name: Flap endonuclease 1
• UniProt Gene Name: Fen1
• UniProt Synonym Gene Names: FEN-1

Uniprot Description

Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.