Rabbit Farnesyl Diphosphate Farnesyltransferase 1 ELISA Kit | FDFT1 elisa kit

Rabbit Farnesyl Diphosphate Farnesyltransferase 1 ELISA Kit

Cat. Num. AAA730133

• Gene Names: FDFT1; SS; SQS; DGPT; ERG9
• Reactivity: Rabbit
• Synonyms: Farnesyl Diphosphate Farnesyltransferase 1; Rabbit Farnesyl Diphosphate Farnesyltransferase 1 ELISA Kit; FDFT1 elisa kit

• Reactivity: Rabbit

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 5.0-100 U/L
• Sensitivity: 1.0u/L
• Intended Uses: This FDFT1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit FDFT1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FDFT1 elisa kit

Principle of the Assay: FDFT1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FDFT1 antibody and an FDFT1-HRP conjugate. The assay sample and buffer are incubated together with FDFT1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FDFT1 concentration since FDFT1 from samples and FDFT1-HRP conjugate compete for the anti-FDFT1 antibody binding site. Since the number of sites is limited, as more sites are occupied by FDFT1 from the sample, fewer sites are left to bind FDFT1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FDFT1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for FDFT1 elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 48146183
• NCBI GeneID: 2222
• UniProt Accession #: Q6IAX1; P37268
• Molecular Weight: 48,115 Da
• NCBI Official Full Name: FDFT1
• NCBI Official Synonym Full Names: farnesyl-diphosphate farnesyltransferase 1
• NCBI Official Symbol: FDFT1
• NCBI Official Synonym Symbols: SS; SQS; DGPT; ERG9
• NCBI Protein Information: squalene synthase; squalene synthetase; FPP:FPP farnesyltransferase; presqualene-di-diphosphate synthase
• UniProt Protein Name: FDFT1 protein
• UniProt Gene Name: FDFT1
• UniProt Synonym Gene Names: hCG_17749
• UniProt Entry Name: Q6IAX1_HUMAN

NCBI Description

This gene encodes a membrane-associated enzyme located at a branch point in the mevalonate pathway. The encoded protein is the first specific enzyme in cholesterol biosynthesis, catalyzing the dimerization of two molecules of farnesyl diphosphate in a two-step reaction to form squalene. [provided by RefSeq, Jul 2008]

Uniprot Description

FDFT1: a membrane-associated enzyme located at a branch point in the mevalonate pathway. The encoded protein is the first specific enzyme in cholesterol biosynthesis, catalyzing the dimerization of two molecules of farnesyl diphosphate in a two-step reaction to form squalene. [provided by RefSeq, Jul 2008]

Protein type: Membrane protein, multi-pass; Transferase; Oxidoreductase; Lipid Metabolism - steroid biosynthesis; EC 2.5.1.21; Membrane protein, integral; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 8p23.1-p22

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane

Molecular Function: farnesyl-diphosphate farnesyltransferase activity; oxidoreductase activity

Biological Process: isoprenoid biosynthetic process; farnesyl diphosphate metabolic process; cellular lipid metabolic process; steroid biosynthetic process; cholesterol biosynthetic process