Apo Transferrin (ATF) Active Protein | ATF active protein
Human Apo Transferrin (ATF)
Reactivity
Human
Purity
Greater than 98.0% as determined by Cellulose Acetate Electrophoresis.
Synonyms
Apo Transferrin (ATF); Human Apo Transferrin (ATF); ATF active protein
Host
FDA approved Plasma
Reactivity
Human
Purity/Purification
Greater than 98.0% as determined by Cellulose Acetate Electrophoresis.
Form/Format
The protein (1mg/ml) was lyophilized from approximately 0.02M NH4HCO3. May contain traces of buffer salts.
Human Virus Test
FDA approved Plasma from each donor has been tested and found negative for antibody to HIV-1, HIV-2, HCV, HBSAG, HBc, ALT and Syphilis. Viral inactivation by pasteurization (60°C for 10 hours) has been validated using three different test viruses, with removal of 8-14.5 logs of virus documented. The purification process has also been found to remove significant additional quantities of virus.
Biological Activity/Protein Uses
Human Transferrin is a crucial component for the cultivation and long-term cells growth of mammalian cells in-vitro. It is used as detoxificant in media by binding contaminating metal ion and is often used as a nutrient in fermentation media for recombinant protein and biopharmaceutical production. Additional common uses of Human Transferrin are: * Molecular weight, Affinity purification of anti-human transferrin antibodies. * Receptor mediated transfection of molecules such as DNA, into cells.
Reconstitution
It is recommended to reconstitute the lyophilized Apo Transferin in sterile 18M omega-cm H2O n less than than 100 ug/mL, which can be further diluted to other aqueous solutions.
Preparation and Storage
Lyophilized Apo Transferrin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Apo Transferrin should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Related Product Information for ATF active protein
Transferrin is the iron-transport protein of vertebrate serum and donates iron to cells through interaction with a specific membrane receptor, CD71. Transferrin appears to be indispensable for most cells growing in tissue culture.
It is referred to frequently as a growth factor because, in analogy to other growth factor-receptor interactions, proliferating cells express high numbers of transferrin receptors, and the binding of transferrin to their receptors is needed for cells to initiate and maintain their DNA synthesis. Apart from its role as an iron transport protein transferrin acts as a cytokine and has functions that may not be related to its iron-carrying capacity.
It is referred to frequently as a growth factor because, in analogy to other growth factor-receptor interactions, proliferating cells express high numbers of transferrin receptors, and the binding of transferrin to their receptors is needed for cells to initiate and maintain their DNA synthesis. Apart from its role as an iron transport protein transferrin acts as a cytokine and has functions that may not be related to its iron-carrying capacity.
Product Categories/Family for ATF active protein
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Product Notes
The ATF (Catalog #AAA555416) is an Active Protein produced from FDA approved Plasma and is intended for research purposes only. The product is available for immediate purchase. The Human Apo Transferrin (ATF) reacts with Human and may cross-react with other species as described in the data sheet. It is sometimes possible for the material contained within the vial of "Apo Transferrin (ATF), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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