Formation of crista junctions protein 1 (FCJ1) Recombinant Protein | FCJ1 recombinant protein

Recombinant Saccharomyces cerevisiae Formation of crista junctions protein 1 (FCJ1)

• Gene Names: FCJ1; AIM28; FMP13
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Formation of crista junctions protein 1 (FCJ1); Recombinant Saccharomyces cerevisiae Formation of crista junctions protein 1 (FCJ1); Recombinant Formation of crista junctions protein 1 (FCJ1); Formation of crista junctions protein 1; Altered inheritance of mitochondria protein 28 Found in mitochondrial proteome protein 13 Mitofilin; FCJ1 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 18-540
• Sequence: ASINTGTTVASKKASHKFRNTLWTIALSATAFYAGGIIYSQKNDKFGDFFSNNVPFAEDLLETYEHYHDRPTLFLEDSWDGLKAKSNDLLSGLTGSSQTRRSNRENIEVKKILSLEPLNIETENSDPQLKEIIGSLNDLINSLNDSNLSIPESEFNSIKKSNQNMLTNLSQLNETLKEALSNYMIQRTSEVITELNTQYENSKREFEKNLQKNLLQEVDEFKENLTKQKDKELEEKLKANEELLQAKHANEVGLLSITQVKEFNKIIKDKIEKERNGRLAHLEEINSEVNDLSKSIDRSSKILSKNEALVQLTFQVDEIKSRINNNNLPDVNIDKELSRLKLLSNLLSTFNKKSCCDDGDCCSCKKGNKNEGKEGKISCKCKPKTNPPSLLSVALDELESTCSGKKILSNEQIYNRWNLLADDFKTASLLPPNSGILGQLTAKVFSLFLFTKTGNPSNATDFDSVYARVGDNLRVSNLNDAVEEVVSLKGWPHKVCESWIEDARRKLEVQRLVEILDCEIRTL
• Sequence Length: 540

• Species: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
• Preparation and Storage: Store at -20 degree C. For extended storage, store at -20 or -80 degree C.

NCBI and Uniprot Product Information

• NCBI GI #: 6322868
• NCBI GeneID: 853886
• NCBI Accession #: NP_012941.1
• NCBI GenBank Nucleotide #: NM_001179806.1
• UniProt Accession #: P36112; D6VX81
• Molecular Weight: 61,082 Da
• NCBI Official Full Name: Fcj1p
• NCBI Official Symbol: FCJ1
• NCBI Official Synonym Symbols: AIM28; FMP13
• NCBI Protein Information: Fcj1p
• UniProt Protein Name: Formation of crista junctions protein 1
• UniProt Gene Name: FCJ1
• UniProt Synonym Gene Names: AIM28; FMP13
• UniProt Entry Name: FCJ1_YEAST

Uniprot Description

Function: Multifunctional regulator of mitochondrial architecture and protein biogenesis. May act in concert with ATP synthase dimers to generate inner membrane structure and consequently normal mitochondrial morphology. Plays a role in keeping cristae membranes connected to the inner boundary membrane. Promotes protein import via the mitochondrial intermembrane space assembly (MIA) pathway. Ref.6 Ref.8 Ref.9

Subunit structure: Core component of the large mitochondrial inner membrane organizing system (MINOS/MitOS) complex, composed of FCJ1, AIM5, AIM13, AIM37, MOS1 and MOS2. Interacts with TOM22 and TOM40 in a MINOS/MitOS-independent manner, resulting in coupling with the outer membrane. Interacts with MIA40, OM45 and POR1. Ref.8 Ref.9

Subcellular location: Mitochondrion inner membrane; Single-pass membrane protein. Note: Concentrates at mitochondrial cristae junctions. Ref.3 Ref.5 Ref.6 Ref.8 Ref.9

Disruption phenotype: Increases frequency of mitochondrial genome loss and leads to altered mitochondrial morphology. Moderately reduces mitochondrial membrane potential and import efficiency of preproteins that are transported into or across the inner membrane via TIM23 complex. Impairs precursor protein import via the MIA pathway. 60-70% of mitochondria exhibit an increased inner membrane surface and stacks of lamellar cristae disconnected from the inner boundary membrane. Ref.7 Ref.8 Ref.9

Miscellaneous: Present with 5730 molecules/cell in log phase SD medium.

Sequence similarities: Belongs to the FCJ1 family.