F-box/LRR-repeat protein 2 Recombinant Protein | FBXL2 recombinant protein

Recombinant Human F-box/LRR-repeat protein 2

Cat. Num. AAA9421477

• Gene Names: FBXL2; FBL2; FBL3
• Reactivity: Human
• Purity: Greater than 90% as determined by SDS-PAGE.
• Synonyms: F-box/LRR-repeat protein 2; Recombinant Human F-box/LRR-repeat protein 2; FBXL2 recombinant protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: Greater than 90% as determined by SDS-PAGE.
• Form/Format: 20mM Tris-HCl based buffer, pH8.0
• Sequence Positions: Full Length, 1-423aa

• Tag Info: His-SUMO-tag
• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C. Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.

NCBI and Uniprot Product Information

• NCBI GI #: 284447314
• NCBI GeneID: 25827
• NCBI Accession #: NP_001165184.1
• NCBI GenBank Nucleotide #: NM_001171713.2
• UniProt Accession #: Q9UKC9; Q6IAN3; Q9NVQ8; Q9UK27; Q9UKA5; Q9Y3Y9; B4DQV0; E9PD06
• Molecular Weight: 63.03kD
• NCBI Official Full Name: F-box/LRR-repeat protein 2 isoform 2
• NCBI Official Synonym Full Names: F-box and leucine rich repeat protein 2
• NCBI Official Symbol: FBXL2
• NCBI Official Synonym Symbols: FBL2; FBL3
• NCBI Protein Information: F-box/LRR-repeat protein 2
• UniProt Protein Name: F-box/LRR-repeat protein 2
• Protein Family: F-box/LRR-repeat protein
• UniProt Gene Name: FBXL2

NCBI Description

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains 12 tandem leucine-rich repeats. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010]

Uniprot Description

FBXL2: Calcium-activated substrate recognition component of a SCF (SKP1-cullin-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Unlike many F-box proteins, FBXL2 doesn't seem to target phosphodegron within its substrates but rather calmodulin-binding motifs. Targets PCYT1A for its monoubiquitination and degradation, this is antagonized by calmodulin. Targets the cyclins CCND2 and CCND3 for polyubiquitination and degradation, leading to cell-cycle arrest in G(0), also antagonized by calmodulin. Binds to hepatitis C virus non-structural protein 5A (NS5A) in a reaction crucial for hepatitis C virus RNA replication.

Protein type: Ubiquitin ligase

Chromosomal Location of Human Ortholog: 3p22.3

Cellular Component: cytoplasm; membrane; SCF ubiquitin ligase complex

Molecular Function: protein binding; protein phosphatase binding; ubiquitin-protein ligase activity

Biological Process: protein modification process; protein ubiquitination; proteolysis; regulation of autophagy; regulation of phosphoinositide 3-kinase cascade; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process