FBXL2 Blocking Peptide | FBXL2 (HGNC:13598) blocking peptide

FBXL2 Antibody (C-term) Blocking Peptide

• Gene Names: FBXL2; FBL2; FBL3
• Synonyms: FBXL2; FBXL2 Antibody (C-term) Blocking Peptide; F-box/LRR-repeat protein 2; F-box and leucine-rich repeat protein 2 ; F-box protein FBL2/FBL3; FBXL2 (HGNC:13598) blocking peptide

• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 423

• Cellular Location: Membrane; Lipid-anchor
• Tissue Location: Expressed in brain, heart, kidney, liver, lung, pancreas and placenta.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for FBXL2 (HGNC:13598) blocking peptide

Calcium-activated substrate recognition component of the SCF (SKP1-cullin-F-box protein) E3 ubiquitin-protein ligase complex, SCF(FBXL2), which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Unlike many F-box proteins, FBXL2 does not seem to target phosphodegron within its substrates but rather calmodulin-binding motifs and is thereby antagonized by calmodulin. This is the case for the cyclins CCND2 and CCND3 which polyubiquitination and subsequent degradation are inhibited by calmodulin. Through CCND2 and CCND3 degradation may induce cell-cycle arrest in G(0). SCF(FBXL2) may also mediate PIK3R2 ubiquitination and proteasomal degradation thereby regulating phosphatidylinositol 3-kinase signaling and indirectly autophagy (PubMed:22323446, PubMed:22020328, PubMed:23604317). PCYT1A monoubiquitination by SCF(FBXL2) and subsequent degradation may regulate synthesis of phosphatidylcholine, which is utilized for formation of membranes and of pulmonary surfactant (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 145559475
• NCBI GeneID: 25827
• UniProt Accession #: Q9UKC9; Q6IAN3; Q9NVQ8; Q9UK27; Q9UKA5; Q9Y3Y9; B4DQV0; E9PD06
• Molecular Weight: 39,613 Da
• NCBI Official Full Name: F-box/LRR-repeat protein 2
• NCBI Official Synonym Full Names: F-box and leucine rich repeat protein 2
• NCBI Official Symbol: FBXL2
• NCBI Official Synonym Symbols: FBL2; FBL3
• NCBI Protein Information: F-box/LRR-repeat protein 2
• UniProt Protein Name: F-box/LRR-repeat protein 2
• UniProt Gene Name: FBXL2
• UniProt Entry Name: FBXL2_HUMAN

NCBI Description

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains 12 tandem leucine-rich repeats. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010]

Uniprot Description

FBXL2: Calcium-activated substrate recognition component of a SCF (SKP1-cullin-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Unlike many F-box proteins, FBXL2 doesn't seem to target phosphodegron within its substrates but rather calmodulin-binding motifs. Targets PCYT1A for its monoubiquitination and degradation, this is antagonized by calmodulin. Targets the cyclins CCND2 and CCND3 for polyubiquitination and degradation, leading to cell-cycle arrest in G(0), also antagonized by calmodulin. Binds to hepatitis C virus non-structural protein 5A (NS5A) in a reaction crucial for hepatitis C virus RNA replication.

Protein type: Ubiquitin ligase

Chromosomal Location of Human Ortholog: 3p22.3

Cellular Component: cytoplasm; membrane; SCF ubiquitin ligase complex

Molecular Function: protein binding; protein phosphatase binding; ubiquitin-protein ligase activity

Biological Process: protein modification process; protein ubiquitination; proteolysis; regulation of autophagy; regulation of phosphoinositide 3-kinase cascade; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process