Antigen 85A Recombinant Protein | fbpA recombinant protein

Antigen 85A, 43-338aa, Mycobacterium tuberculosis, His tag, Baculovirus

Cat. Num. AAA204142

• Gene Names: fbpA; 85A; mpt44
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: Antigen 85A; 43-338aa; Mycobacterium tuberculosis; His tag; Baculovirus; Antigen 85 complex A; Mycolytransferase 85A; Fibronectin-binding protein A; TB Ab85A; TBab85A; fbpA recombinant protein

• Host: Baculovirus
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing, 10% glycerol
• Concentration: 0.5 mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: ADPAFSRPGL PVEYLQVPSP SMGRDIKVQF QSGGANSPAL YLLDGLRAQD DFSGWDINTP AFEWYDQSGL SVVMPVGGQS SFYSDWYQPA CGKAGCQTYK WETFLTSELP GWLQANRHVK PTGSAVVGLS MAASSALTLA IYHPQQFVYA GAMSGLLDPS QAMGPTLIGL AMGDAGGYKA SDMWGPKEDP AWQRNDPLLN VGKLIANNTR VWVYCGNGKP SDLGGNNLPA KFLEGFVRTS NIKFQDAYNA GGGHNGVFDF PDSGTHSWEY WGAQLNAMKP DLQRALGATP NTGPAPQGAH HHHHH
• Sequence Length: 338

• Applicable Applications for fbpA recombinant protein: SDS-PAGE
• Antigen Species: Mycobacterium tuberculosis
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for fbpA recombinant protein

Antigen 85A, belong to the antigen 85 complex (Antigen 85A, B, C). The enzymes of the antigen 85 complex possess mycolyltransferase activity and catalyze the synthesis of the most abundant glycolipid of the mycobacterial cell wall, the cord factor. The cord factor (trehalose 6,6'-dimycolate, TDM) is essential for the integrity of the mycobacterial cell wall and pathogenesis of the bacillus. TDM is synthesized from two molecules of trehalose-6'-monomycolate (TMM) by Antigen 85A. Recombinant Mycobacterium tuberculosis Antigen 85A protein, fused to His-tag at C-terminus, was expressed in Hi-5 cell and purified by using conventional chromatography techniques.

References

Elamin AA., et al. (2009) J Microbiol Methods. 79 (3):358-63.; Jeon BY., et al. (2011) Microbes Infect. 13 (3):284-90.

NCBI and Uniprot Product Information

• NCBI GI #: 15610940
• NCBI GeneID: 886132
• NCBI Accession #: NP_218321.1
• NCBI GenBank Nucleotide #: NC_000962.3
• Molecular Weight: 32.8kDa (305aa)
• NCBI Official Full Name: diacylglycerol acyltransferase/mycolyltransferase Ag85A
• NCBI Official Symbol: fbpA
• NCBI Official Synonym Symbols: 85A; mpt44
• NCBI Protein Information: diacylglycerol acyltransferase/mycolyltransferase Ag85A
• UniProt Protein Name: Diacylglycerol acyltransferase/mycolyltransferase Ag85A
• Protein Family: Fe(3+)-binding periplasmic protein
• UniProt Gene Name: fbpA
• UniProt Synonym Gene Names: mpt44; DGAT; 85A; Ag85A; Fbps A

Uniprot Description

The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs). They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan, and through the synthesis of alpha,alpha-trehalose dimycolate (TDM, cord factor). They catalyze the transfer of a mycoloyl residue from one molecule of alpha,alpha-trehalose monomycolate (TMM) to another TMM, leading to the formation of TDM. FbpA mediates triacylglycerol (TAG) formation with long-chain acyl-CoA as the acyl donor and 1,2-dipalmitoyl-sn-glycerol (1,2-dipalmitin) as the acyl acceptor. It has a preference for C26:0-CoA over C18:1-CoA.