Sheep Fibrillin 2 ELISA Kit | FBN2 elisa kit

Sheep Fibrillin 2 ELISA Kit

Cat. Num. AAA752906

• Gene Names: Fbn2; sy; sne; Fib-2; BC063774; mKIAA4226
• Reactivity: Sheep
• Synonyms: Fibrillin 2; Sheep Fibrillin 2 ELISA Kit; FBN2 elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Intended Uses: This FBN2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep FBN2. This ELISA kit for research use only, not for therapeutic applications
• !Intended Uses: This FBN2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep FBN2. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FBN2 elisa kit

Principle of the Assay: FBN2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FBN2 antibody and an FBN2-HRP conjugate. The assay sample and buffer are incubated together with FBN2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FBN2 concentration since FBN2 from samples and FBN2-HRP conjugate compete for the anti-FBN2 antibody binding site. Since the number of sites is limited, as more sites are occupied by FBN2 from the sample, fewer sites are left to bind FBN2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FBN2 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for FBN2 elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 118136302
• NCBI GeneID: 14119
• NCBI Accession #: NP_034311.2
• NCBI GenBank Nucleotide #: NM_010181.2
• Molecular Weight: 313,818 Da
• NCBI Official Full Name: fibrillin-2
• NCBI Official Synonym Full Names: fibrillin 2
• NCBI Official Symbol: Fbn2
• NCBI Official Synonym Symbols: sy; sne; Fib-2; BC063774; mKIAA4226
• NCBI Protein Information: fibrillin-2; syndatyly ems
• UniProt Protein Name: Fibrillin-2
• Protein Family: Fibrillin
• UniProt Gene Name: Fbn2
• UniProt Synonym Gene Names: Fbn-2
• UniProt Entry Name: FBN2_MOUSE

Uniprot Description

FBN2: Fibrillins are structural components of 10-12 nm extracellular calcium-binding microfibrils, which occur either in association with elastin or in elastin-free bundles. Fibrillin-2- containing microfibrils regulate the early process of elastic fiber assembly. Regulates osteoblast maturation by controlling TGF-beta bioavailability and calibrating TGF-beta and BMP levels, respectively. Defects in FBN2 are the cause of distal arthrogryposis type 9 (DA9). A form of distal arthrogryposis, a disease characterized by congenital joint contractures that mainly involve two or more distal parts of the limbs, in the absence of a primary neurological or muscle disease. DA9 is a connective tissue disorder characterized by contractures, arachnodactyly, scoliosis, and crumpled ears. Belongs to the fibrillin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; microfibril; extracellular region

Molecular Function: extracellular matrix structural constituent; calcium ion binding

Biological Process: positive regulation of osteoblast differentiation; positive regulation of bone mineralization; limb morphogenesis; embryonic limb morphogenesis