Human Fibulin-2 ELISA Kit | FBLN2 elisa kit

Human Fibulin-2 ELISA Kit

Cat. Num. AAA760401

• Reactivity: Human
• Synonyms: Fibulin-2; Human Fibulin-2 ELISA Kit; FBLN2/Fibulin-2/FIBL-2; FBLN2 elisa kit

• Reactivity: Human
• Sequence Length: 1231

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 15.6-1000pg/ml
• Sensitivity: 9.375pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for FBLN2 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human FBLN2antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- Human FBLN2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human FBLN2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human FBLN2can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 259013547
• NCBI GeneID: 2199
• NCBI Accession #: NP_001158507.1
• NCBI GenBank Nucleotide #: NM_001165035.1
• Molecular Weight: 131,863 Da
• NCBI Official Full Name: fibulin-2 isoform a
• NCBI Official Synonym Full Names: fibulin 2
• NCBI Official Symbol: FBLN2
• NCBI Protein Information: fibulin-2
• UniProt Protein Name: Fibulin-2
• Protein Family: Fibulin
• UniProt Gene Name: FBLN2
• UniProt Synonym Gene Names: FIBL-2
• UniProt Entry Name: FBLN2_HUMAN

NCBI Description

This gene encodes an extracellular matrix protein, which belongs to the fibulin family. This protein binds various extracellular ligands and calcium. It may play a role during organ development, in particular, during the differentiation of heart, skeletal and neuronal structures. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

FBLN2: Its binding to fibronectin and some other ligands is calcium dependent. Belongs to the fibulin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 3p25.1

Cellular Component: extracellular matrix; extracellular region; proteinaceous extracellular matrix

Molecular Function: calcium ion binding; extracellular matrix binding; extracellular matrix structural constituent

Biological Process: extracellular matrix organization and biogenesis