Filamin Binding LIM Protein 1 Recombinant Protein | FBLIM1 recombinant protein

Recombinant Human Filamin Binding LIM Protein 1

Cat. Num. AAA146448

• Gene Names: FBLIM1; CAL; FBLP1; FBLP-1
• Purity: Greater than 85.0% as determined by SDS-PAGE.
• Synonyms: Filamin Binding LIM Protein 1; Recombinant Human Filamin Binding LIM Protein 1; FBLIM1 Human; Filamin Binding LIM Protein 1 Human Recombinant; Filamin binding LIM protein 1; CAL; FBLP-1; FBLP1; RP11-169K16.5; Migfilin; Mitogen-inducible 2-interacting protein; MIG2-interacting protein; FBLIM1 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 85.0% as determined by SDS-PAGE.
• Form/Format: The FBLIM1 solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.; Sterile Filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMASKPEK RVASSVFITL APPRRDVAVA EEVRQAVCEA RRGRPWEAPA PMKTPEAGLA GRPSPWTTPG RAAATVPAAP MQLFNGGCPP PPPVLDGEDV LPDLDLLPPP PPPPPVLLPS EEEAPAPMGA SLIADLEQLH LSPPPPPPQA PAEGPSVQPG PLRPMEEELP PPPAEPVEKG ASTDICAFCH KTVSPRELAV EAMKRQYHAQ CFTCRTCRRQ LAGQSFYQKD GRPLCEPCYQ DTLERCGKCG EVVRDHIIRA LGQAFHPSCF TCVTCARCIG DESFALGSQN EVYCLDDFYR KFAPVCSICE NPIIPRDGKD AFKIECMGRN FHENCYRCED CRILLSVEPT DQGCYPLNNH LFCKPCHVKR SAAGCC.
• Sequence Length: 374

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Related Product Information for FBLIM1 recombinant protein

Description: FBLIM1 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 396 amino acids (1-373) and having a molecular mass of 43.1 kDa. FBLIM1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Introduction: Filamin binding LIM protein 1 (FBLIM1) plays a role as an anchoring site for cell-ECM adhesion proteins and filamin-containing actin filaments. FBLIM1 is involved in cell shape spreading and motility. FBLIM1 participates in the regulation of filamin-mediated cross-linking and stabilization of actin filaments. FBLIM1 promotes stimulation of integrins and regulates integrin-mediated cell-cell adhesion.

Product Categories/Family for FBLIM1 recombinant protein

Recombinant & Natural Proteins; Recombinant Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 66932986
• NCBI GeneID: 54751
• NCBI Accession #: NP_001019386.1
• NCBI GenBank Nucleotide #: NM_001024215.1
• UniProt Accession #: Q8WUP2; Q5VVE0; Q5VVE1; Q8IX23; Q96T00; Q9UFD6; B3KNY0
• Molecular Weight: 30,770 Da
• NCBI Official Full Name: filamin-binding LIM protein 1 isoform b
• NCBI Official Synonym Full Names: filamin binding LIM protein 1
• NCBI Official Symbol: FBLIM1
• NCBI Official Synonym Symbols: CAL; FBLP1; FBLP-1
• NCBI Protein Information: filamin-binding LIM protein 1; CSX-associated LIM; MIG2-interacting protein; filamin-binding LIM protein-1; migfilin; mitogen-inducible 2 interacting protein; mitogen-inducible 2-interacting protein
• UniProt Protein Name: Filamin-binding LIM protein 1
• Protein Family: Filamin-binding LIM protein
• UniProt Gene Name: FBLIM1
• UniProt Synonym Gene Names: FBLP1; FBLP-1; MIG2-interacting protein
• UniProt Entry Name: FBLI1_HUMAN

NCBI Description

This gene encodes a protein with an N-terminal filamin-binding domain, a central proline-rich domain, and, multiple C-terminal LIM domains. This protein localizes at cell junctions and may link cell adhesion structures to the actin cytoskeleton. This protein may be involved in the assembly and stabilization of actin-filaments and likely plays a role in modulating cell adhesion, cell morphology and cell motility. This protein also localizes to the nucleus and may affect cardiomyocyte differentiation after binding with the CSX/NKX2-5 transcription factor. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

FBLIM1: Serves as an anchoring site for cell-ECM adhesion proteins and filamin-containing actin filaments. Is implicated in cell shape modulation (spreading) and motility. May participate in the regulation of filamin-mediated cross-linking and stabilization of actin filaments. May also regulate the assembly of filamin- containing signaling complexes that control actin assembly. Promotes dissociation of FLNA from ITGB3 and ITGB7. Promotes activation of integrins and regulates integrin-mediated cell-cell adhesion. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1p36.21

Cellular Component: focal adhesion; stress fiber; cell cortex; cytosol

Molecular Function: zinc ion binding; filamin binding

Biological Process: regulation of cell shape; cell-cell adhesion; regulation of integrin activation