Fibrillarin Recombinant Protein | FBL recombinant protein

Recombinant human Fibrillarin

Cat. Num. AAA144198

• Gene Names: FBL; FIB; FLRN; RNU3IP1
• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Synonyms: Fibrillarin; Recombinant human Fibrillarin; rRNA 2'-O-methyltransferase fibrillarin; 34 kDa nucleolar scleroderma antigen; FBL; FIB1; FLRN; fibrillarin; FIB; RNU3IP1.; FBL recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 90.0% as determined by SDS-PAGE.
• Form/Format: The FBL solution (0.5 mg/ml) contains Phosphate buffered saline (pH 7.4), 30% glycerol and 1 mM EDTA.; Sterile Filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH RSMGKNVMVE PHRHEGVFIC RGKEDALVTK NLVPGESVYG EKRVSISEGD DKIEYRAWNP FRSKLAAAIL GGVDQIHIKP GAKVLYLGAA SGTTVSHVSD IVGPDGLVYA VEFSHRSGRD LINLAKKRTN IIPVIEDARH PHKYRMLIAM VDVIFADVAQ PDQTRIVALN AHTFLRNGGH FVISIKANCI DSTASAEAVF ASEVKKMQQE NMKPQEQLTL EPYERDHAVV VGVYRPPPKV KN
• Sequence Length: 321

• Preparation and Storage: Store at 4°C if entire viall will be used within 2-4 weeks.; Store frozen at -20°C for longer periods of time.; For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). ; ; Avoid multiple freeze-thaw cycles.

Related Product Information for FBL recombinant protein

Description: FBL Human Recombinant fused with 23 amino acid His tag at N-terminus produced in E Coli is a single, non-glycosylated, polypeptide chain containing 262 amino acids (83-321 a.a.) and having a molecular mass of 28.9kDa. The FBL is purified by proprietary chromatographic techniques.

Introduction: FBL is a significant small nucleolar protein in eukaryotes, which has an essential role in pre-rRNA processing during ribosomal biogenesis. Fibrillarin is a component of several ribonucleoproteins including a nucleolar small nuclear ribonucleoprotein (SnRNP) and one of the two classes of small nucleolar ribonucleoproteins (snoRNPs). Fibrillarin contains an N-terminal repetitive domain which is rich in glycine and arginine residues, like fibrillarins in other species. Fibrillarin's central region is similar to an RNA-binding domain and contains an RNP consensus sequence. FBL is linked to the U3, U8, and U13 small nuclear RNAs and is positioned in the dense fibrillar component (DFC) of the nucleolus. Antisera from roughly 8% of humans with the autoimmune disease scleroderma recognize fibrillarin.

Product Categories/Family for FBL recombinant protein

Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 12056465
• NCBI GeneID: 2091
• NCBI Accession #: NP_001427.2
• NCBI GenBank Nucleotide #: NM_001436.3
• UniProt Accession #: P22087; O75259; Q6IAT5; Q9UPI6; B5BUE8
• NCBI Official Full Name: rRNA 2'-O-methyltransferase fibrillarin
• NCBI Official Synonym Full Names: fibrillarin
• NCBI Official Symbol: FBL
• NCBI Official Synonym Symbols: FIB; FLRN; RNU3IP1
• NCBI Protein Information: rRNA 2'-O-methyltransferase fibrillarin; 34 kDa nucleolar scleroderma antigen; 34-kD nucleolar scleroderma antigen; RNA, U3 small nucleolar interacting protein 1; histone-glutamine methyltransferase
• UniProt Protein Name: rRNA 2'-O-methyltransferase fibrillarin
• Protein Family: Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase
• UniProt Gene Name: FBL
• UniProt Synonym Gene Names: FIB1; FLRN
• UniProt Entry Name: FBRL_HUMAN

NCBI Description

This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin. [provided by RefSeq, Jul 2008]

Uniprot Description

FBL: Involved in pre-rRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'- hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Interacts with NOLC1. Component of box C/D small nucleolar ribonucleoprotein (snoRNP) particles that contain NHP2L1, FBL, NOP5 and NOP56, plus a guide RNA. It is associated with the U3, U8, U13, X and Y small nuclear RNAs. Component of several ribosomal and nucleolar protein complexes. Interacts with PRMT5 and UTP20. Interacts with DDX5. Belongs to the methyltransferase superfamily. Fibrillarin family.

Protein type: RNA processing; Methyltransferase; RNA-binding; Nucleolus; EC 2.1.1.-

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: Cajal body; granular component; membrane; nucleolus; nucleus

Molecular Function: protein binding; RNA binding

Biological Process: osteoblast differentiation; tRNA processing; snoRNA metabolic process; rRNA processing