Factor Related Apoptosis (FAS) Recombinant Protein | FAS recombinant protein

Recombinant Factor Related Apoptosis (FAS)

Cat. Num. AAA2011282

• Gene Names: Fas; Tnfrsf6
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Factor Related Apoptosis (FAS); Recombinant Factor Related Apoptosis (FAS); FAS recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGS-G TPPGKAFNLT WISTDFKTIL EWQPKPTNYT YTVQISDRSR NWKYKCTGTT DTECDLTDEI VKDVNWTYEA RVLSVPWRNS THGKETLFGT HGEEPPFTNA RKFLPYRDTK IGQPVIQKYE QGGTKLKVTV KDSFTLVRKN GTFLTLRQVF GNDLGYILTY RKDSSTGRKT NTTHTNEFLI DVEKGVSYCF FAQAVIFSRK TNHKSPESIT KCTEQWKSVL GE
• Sequence Length: 324

• Applicable Applications for FAS recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Gly30~Glu252 (Accession # Q63199) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 59624977
• NCBI GeneID: 246097
• NCBI Accession #: NP_631933.2
• NCBI GenBank Nucleotide #: NM_139194.2
• UniProt Accession #: Q63199
• Molecular Weight: 26.9kDa
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 6
• NCBI Official Synonym Full Names: Fas cell surface death receptor
• NCBI Official Symbol: Fas
• NCBI Official Synonym Symbols: Tnfrsf6
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 6; CD95 antigen; Fas receptor; apo-1 antigen; FASLG receptor; Fas antigen (ATP1); apoptosis-mediating surface antigen FAS; Fas (TNF receptor superfamily, member 6); Tumor necrosis factor receptor superfamily, member 6
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 6
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: Fas
• UniProt Synonym Gene Names: Apt1; Tnfrsf6
• UniProt Entry Name: TNR6_RAT

NCBI Description

Tnfsf6/Fasl receptor [RGD, Feb 2006]

Uniprot Description

Function: Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both

By similarity.

Subunit structure: Binds DAXX and RIPK1. Interacts with HIPK3. Part of a complex containing HIPK3 and FADD. Interacts with BRE and FEM1B

By similarity. Interacts with FADD

By similarity.

Subcellular location: Membrane; Single-pass type I membrane protein.

Domain: Contains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins.

Sequence similarities: Contains 1 death domain.Contains 3 TNFR-Cys repeats.