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SDS-Page

Family With Sequence Similarity 3 Recombinant Protein | FAM3D recombinant protein

Recombinant Family With Sequence Similarity 3, Member D (FAM3D)

Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
>95%
Synonyms
Family With Sequence Similarity 3; Recombinant Family With Sequence Similarity 3; Member D (FAM3D); FAM3D recombinant protein
Ordering
For Research Use Only!
Host
Host: E Coli
Source: Prokaryotic expression
Purity/Purification
>95%
Form/Format
Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Concentration
9.2 (varies by lot)
Sequence
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- YMSFS MKTIRLPRWL AASPTKEIQV KKYKCGLIKP CPANYFAFKI CSGAANVVGP TMCFEDRMIM SPVKNNVGRG LNIALVNGTT GAVLGQKAFD MYSGDVMHLV KFLKEIPGGA LVLVASYDDP GTKMNDESRK LFSDLGSSYA KQLGFRDSWV FIGAKDLRGK SPFEQFLKNS PDTNKYEGWP ELLEMEGCMPPKPF
Sequence Length
224
Applicable Applications for FAM3D recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Predicted Molecular Mass
25.9kDa
Accurate Molecular Mass (KD)
26kDa
Endotoxin
<1.0EU per 1ug (determined by the LAL method)
Expression System
Prokaryotic expression
Tag
N-terminal His-Tag, T7 tag
Organism Species
Homo sapiens (Human)
Fragment
Tyr26~Phe224
Usage
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

SDS-Page

NCBI and Uniprot Product Information

NCBI GI #
Molecular Weight
24,963 Da
NCBI Official Full Name
FAM3D
UniProt Protein Name
Protein FAM3D
Protein Family
UniProt Gene Name
FAM3D
UniProt Entry Name
FAM3D_HUMAN

Uniprot Description

FAM3D: Belongs to the FAM3 family.

Protein type: Cytokine; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 3p14.2

Cellular Component: extracellular region

Molecular Function: cytokine activity

Biological Process: negative regulation of insulin secretion

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Product Notes

The FAM3D fam3d (Catalog #AAA2029258) is a Recombinant Protein produced from Host: E Coli Source: Prokaryotic expression and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Family With Sequence Similarity 3 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the FAM3D fam3d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHH H SSGLVPRGSH MASMTGGQQM GRGSEF- YMSFS MKTIRLPRWL AASPTKEIQV KKYKCGLIKP CPANYFAFKI CSGAANVVGP TMCFEDRMIM SPVKNNVGRG LNIALVNGTT GAVLGQKAFD MYSGDVMHLV KFLKEIPGGA LVLVASYDDP GTKMNDESRK LFSDLGSSYA KQLGFRDSWV FIGAKDLRGK SPFEQFLKNS PDTNKYEGWP ELLEMEGCMP PKPF. It is sometimes possible for the material contained within the vial of "Family With Sequence Similarity 3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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