Typical Testing Data/Standard Curve (for reference only)
Sheep Family With Sequence Similarity 3, Member B ELISA Kit | FAM3B elisa kit
Sheep Family With Sequence Similarity 3, Member B ELISA Kit
Reactivity
Sheep
Synonyms
Family With Sequence Similarity 3; Member B; Sheep Family With Sequence Similarity 3; Member B ELISA Kit; FAM3B elisa kit
Reactivity
Sheep
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
0.1 ng/mL.
Intended Uses
This FAM3B ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep FAM3B. This ELISA kit for research use only, not for therapeutic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for FAM3B elisa kit
Principle of the assay: FAM3B ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FAM3B antibody and an FAM3B-HRP conjugate. The assay sample and buffer are incubated together with FAM3B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FAM3B concentration since FAM3B from samples and FAM3B-HRP conjugate compete for the anti-FAM3B antibody binding site. Since the number of sites is limited, as more sites are occupied by FAM3B from the sample, fewer sites are left to bind FAM3B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FAM3B concentration in each sample is interpolated from this standard curve.
Product Categories/Family for FAM3B elisa kit
NCBI and Uniprot Product Information
Uniprot Description
FAM3B: Induces apoptosis of alpha and beta cells in a dose- and time-dependent manner. Present in insulin secretory granules and likely cosecreted with insulin. Belongs to the FAM3 family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Cytokine; Nuclear envelope; Secreted, signal peptide; Apoptosis; Secreted
Chromosomal Location of Human Ortholog: 21q22.3
Cellular Component: extracellular space; extracellular region
Molecular Function: cytokine activity
Biological Process: apoptosis; insulin secretion
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Product Notes
The Sheep FAM3B fam3b (Catalog #AAA747174) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA747174 ELISA Kit recognizes Sheep FAM3B. It is sometimes possible for the material contained within the vial of "Family With Sequence Similarity 3, Member B, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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