FAM20C / DMP4 recombinant protein

Human FAM20C / DMP4 Protein (His Tag)

Cat. Num. AAA2545197

• Gene Names: FAM20C; RNS; DMP4; G-CK; DMP-4; GEF-CK
• Purity: > 85 % as determined by SDS-PAGE.
• Synonyms: FAM20C / DMP4; Human FAM20C / DMP4 Protein (His Tag); DMP4; FAM20C / DMP4 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 85 % as determined by SDS-PAGE.
• Form/Format: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % glycerol
• Sequence Length: 584

• Application Notes: The recombinant human FAM20C consists 573 amino acids and predicts a molecular mass of 65.1 kDa.
• Predicted N Terminal: Leu 23
• Endotoxin: <1.0 EU per mug protein as determined by the LAL method.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for FAM20C / DMP4 recombinant protein

A DNA sequence encoding the human FAM20C (NP_064608.2) (Met1-Arg584) was expressed with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 116174742
• NCBI GeneID: 56975
• NCBI Accession #: NP_064608.2
• NCBI GenBank Nucleotide #: NM_020223.3
• UniProt Accession #: Q8IXL6; Q5I0W9; Q7Z4I0; Q9NPT2; A4D2Q5; L8B5W8
• Molecular Weight: 29,597 Da
• NCBI Official Full Name: extracellular serine/threonine protein kinase FAM20C
• NCBI Official Synonym Full Names: FAM20C, golgi associated secretory pathway kinase
• NCBI Official Symbol: FAM20C
• NCBI Official Synonym Symbols: RNS; DMP4; G-CK; DMP-4; GEF-CK
• NCBI Protein Information: extracellular serine/threonine protein kinase FAM20C
• UniProt Protein Name: Extracellular serine/threonine protein kinase FAM20C
• UniProt Gene Name: FAM20C
• UniProt Synonym Gene Names: GEF-CK

NCBI Description

This gene encodes a member of the family of secreted protein kinases. The encoded protein binds calcium and phosphorylates proteins involved in bone mineralization. Mutations in this gene are associated with the autosomal recessive disorder Raine syndrome. [provided by RefSeq, Apr 2014]

Uniprot Description

FAM20C: an atypical serine protein kinase that localizes within the Golgi apparatus and is secreted. Distantly related to the Drosophila Golgi-localized kinase Four-jointed. Phosphorylates secretory proteins including the caseins and members of the small integrin-binding ligand, N-linked glycoprotein (SIBLING) protein family, which modulate biomineralization. Colocalizes with the Golgi resident protein GM130. Preferentially phosphorylates its targets within the S-x-E/pS motif. Requires Mn2+ as an alternative to Mg2+ as the activating cation. Defects in FAM20C are the cause of Raine syndrome (RNS). RNS is an autosomal recessive osteosclerotic bone dysplasia with neonatal lethal outcome. Clinical features include generalized osteosclerosis, craniofacial dysplasia and microcephaly. Two isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7p22.3

Cellular Component: extracellular space; Golgi apparatus

Molecular Function: manganese ion binding; protein binding; protein serine/threonine kinase activity

Biological Process: biomineral formation; protein amino acid phosphorylation

Disease: Raine Syndrome