Fas-Associated Death Domain Recombinant Protein | FADD recombinant protein

Recombinant Human Fas-Associated Death Domain

Cat. Num. AAA143810

• Gene Names: FADD; GIG3; MORT1
• Purity: Greater than 95.0% as determined by SDS-PAGE.
• Synonyms: Fas-Associated Death Domain; Recombinant Human Fas-Associated Death Domain; FADD Human; Fas-Associated Death Domain Human Recombinant; GIG3; MORT1; MGC8528; FADD; Fas (TNFRSF6)-associated via death domain; Protein FADD; FAS-associated death domain protein; FAS-associating death domain-containing protein; Mediator of receptor induced toxicity; Growth-inhibiting gene 3 protein; FADD recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 95.0% as determined by SDS-PAGE.
• Form/Format: The FADD protein solution contains 20mM Tris-HCl, pH-8, and 10% glycerol.; Sterile filtered colorless solution.
• Sequence: MRGSHHHHHHGMASMTGGQQ MGRDLYDDDD KDRWGSMDPF LVLLHSVSSS LSSSELTELK FLCLGRVGKR KLERVQSGLD LFSMLLEQND LEPGHTELLR ELLASLRRHD LLRRVDDFEA GAAAGAAPGE EDLCAAFNVI CDNVGKDWRR LARQLKVSDT KIDSIEDRYP RNLTERVRES LRIWKNTEKE NATVAHLVGA LRSCQMNLVA DLVQEVQQAR DLQNRSGAMS PMSWNSDAST SEAS
• Sequence Length: 208

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Related Product Information for FADD recombinant protein

Description: FADD produced in E Coli is a single, non-glycosylated polypeptide chain containing 244 amino acids (1-208 a.a.) and having a molecular mass of 27.4 kDa.FADD is fused to 36 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Introduction: FADD is an adaptor protein that cooperates with a variety of cell surface receptors and mediates cell apoptotic signals. Using its C-terminal death domain, FADD is recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and consequently it take parts in the death signaling initiated by these receptors. FADD interaction with the receptors reviels the N-terminal effector domain of, which allows it to recruit caspase-8, and thus initiate the cysteine protease cascade. Knockout studies in mice furthermore propose the significance of FADD in premature T cell development. FADD plays a role in survival/proliferation and cell cycle development. FADD also takes part in cellular sublocalization, protein phosphorylation, and inhibitory molecules.

Product Categories/Family for FADD recombinant protein

Recombinant & Natural Proteins; Recombinant Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 4505229
• NCBI GeneID: 8772
• NCBI Accession #: NP_003815.1
• NCBI GenBank Nucleotide #: NM_003824.3
• UniProt Accession #: Q13158; Q14866; Q6IBR4
• Molecular Weight: 23,279 Da
• NCBI Official Full Name: FAS-associated death domain protein
• NCBI Official Synonym Full Names: Fas (TNFRSF6)-associated via death domain
• NCBI Official Symbol: FADD
• NCBI Official Synonym Symbols: GIG3; MORT1
• NCBI Protein Information: FAS-associated death domain protein; Fas-associating death domain-containing protein; Fas-associating protein with death domain; growth-inhibiting gene 3 protein; mediator of receptor induced toxicity; mediator of receptor-induced toxicity
• UniProt Protein Name: FAS-associated death domain protein
• Protein Family: Fas-associated death domain protein
• UniProt Gene Name: FADD
• UniProt Synonym Gene Names: MORT1
• UniProt Entry Name: FADD_HUMAN

NCBI Description

The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development. [provided by RefSeq, Jul 2008]

Uniprot Description

FADD: an adaptor molecule that mediates apoptosis. Recruited through its C-terminal death domain by Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TRAIL-receptor, participating in the death signaling initiated by these receptors. Interaction with the receptors unmasks the N-terminal effector domain of this protein, which recruits caspase-8, and thereby activates the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development.

Protein type: Apoptosis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 11q13.3

Cellular Component: neuron projection; CD95 death-inducing signaling complex; cytosol; lipid raft

Molecular Function: identical protein binding; protein binding; protease binding; death receptor binding; protein complex binding; tumor necrosis factor receptor superfamily binding; tumor necrosis factor receptor binding

Biological Process: viral reproduction; positive regulation of proteolysis; protein heterooligomerization; positive regulation of apoptosis; positive regulation of T cell mediated cytotoxicity; apoptosis; T cell differentiation in the thymus; toll-like receptor 3 signaling pathway; positive regulation of activated T cell proliferation; positive regulation of interleukin-8 production; T cell homeostasis; positive regulation of macrophage differentiation; defense response to virus; toll-like receptor 4 signaling pathway; positive regulation of adaptive immune response; spleen development; caspase activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; thymus development; MyD88-independent toll-like receptor signaling pathway; positive regulation of tumor necrosis factor production; lymph node development; positive regulation of interferon-gamma production; induction of apoptosis via death domain receptors; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter

Disease: Infections, Recurrent, With Encephalopathy, Hepatic Dysfunction, And Cardiovascular Malformations