Fatty Acid Binding Protein 4, Adipocyte (FABP4) Recombinant Protein | FABP4 recombinant protein

Recombinant Fatty Acid Binding Protein 4, Adipocyte (FABP4)

Cat. Num. AAA2010191

• Gene Names: FABP4; AP2; ALBP; AFABP; FABP3; A-FABP
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Fatty Acid Binding Protein 4; Adipocyte (FABP4); Recombinant Fatty Acid Binding Protein 4; FABP4 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGS-CDAFVGTWK LVSSENFDDY MKEVGVGFAT RKVAGMAKPN LIITVNGDMI TIRSESTFKN TEIAFKLGQE FDEVTADDRKVKSTITLDGG ALVQVQKWDG KTTTINRKIV DDKLVVECIM KGVTATRIYE RA
• Sequence Length: 132

• Applicable Applications for FABP4 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Sus scrofa; Porcine (Pig)
• Expression System: Prokaryotic expression
• Residues: Cys2~Ala132 (Accession # O97788) with two N-terminal Tags, His-tag and GST-tag
• Subcellular Location: Cytoplasm. Nucleus.
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 6.3
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 257467617
• NCBI GeneID: 399533
• NCBI Accession #: NP_001002817.1
• NCBI GenBank Nucleotide #: NM_001002817.1
• UniProt Accession #: O97788; A0SXU3; A0SXU5; A0SXV1; A0SXV2; A0SXW5
• Molecular Weight: 46.2kDa
• NCBI Official Full Name: fatty acid-binding protein, adipocyte
• NCBI Official Symbol: FABP4
• NCBI Official Synonym Symbols: AP2; ALBP; AFABP; FABP3; A-FABP
• NCBI Protein Information: fatty acid-binding protein, adipocyte; fatty acid-binding protein 4; adipocyte lipid-binding protein; adipocyte-type fatty acid-binding protein
• UniProt Protein Name: Fatty acid-binding protein, adipocyte
• Protein Family: Fatty acid-binding protein
• UniProt Gene Name: FABP4
• UniProt Synonym Gene Names: ALBP; A-FABP; AFABP
• UniProt Entry Name: FABP4_PIG

Uniprot Description

Function: Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus

By similarity. Involved in the regulation of intramuscular fat accretion.

Subunit structure: Monomer and homodimer. Interacts with PPARG

By similarity.

Subcellular location: Cytoplasm. Nucleus. Note: Depending on the nature of the ligand, a conformation change exposes a nuclear localization motif and the protein is transported into the nucleus. Subject to constitutive nuclear export

By similarity.

Tissue specificity: Adipose tissue. Ref.4

Domain: Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior

By similarity.

Post-translational modification: The N-terminus is blocked.

Sequence similarities: Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.