Human coagulation factor VIII, procoagulant component ELISA Kit | F8 elisa kit

Human coagulation factor VIII, FVIII ELISA Kit

Cat. Num. AAA700550

• Gene Names: F8; AHF; F8B; F8C; HEMA; FVIII; DXS1253E
• Reactivity: Human
• Synonyms: coagulation factor VIII; procoagulant component; Human coagulation factor VIII; FVIII ELISA Kit; Human coagulation factor VIII (FVIII) ELISA Kit; RP11-115M6.7; AHF; DXS1253E; F8B; F8C; FVIII; HEMA; coagulation factor VIIIc; factor VIII F8B; F8 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human FV III. No significant cross-reactivity or interference between human FV III and analogues was observed.
• Sequence Length: 2351

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 12.5 ng/ml-800 ng/ml
• Sensitivity: < 3.12 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for F8 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FV III has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FV III present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FV III is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FV III bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4503647
• NCBI GeneID: 2157
• NCBI Accession #: NP_000123.1
• NCBI GenBank Nucleotide #: NM_000132.3
• UniProt Accession #: P00451; Q14286; Q5HY69
• Molecular Weight: 267,009 Da
• NCBI Official Full Name: coagulation factor VIII isoform a
• NCBI Official Synonym Full Names: coagulation factor VIII, procoagulant component
• NCBI Official Symbol: F8
• NCBI Official Synonym Symbols: AHF; F8B; F8C; HEMA; FVIII; DXS1253E
• NCBI Protein Information: coagulation factor VIII; factor VIII F8B; antihemophilic factor; coagulation factor VIIIc
• UniProt Protein Name: Coagulation factor VIII
• Protein Family: Coagulation factor
• UniProt Gene Name: F8
• UniProt Synonym Gene Names: F8C; AHF
• UniProt Entry Name: FA8_HUMAN

NCBI Description

This gene encodes coagulation factor VIII, which participates in the intrinsic pathway of blood coagulation; factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein, isoform b, which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. Defects in this gene results in hemophilia A, a common recessive X-linked coagulation disorder. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa.