Human Proteinase-activated receptor 4 ELISA Kit | F2RL3 elisa kit

Human Proteinase-activated receptor 4 (F2RL3) ELISA Kit

Cat. Num. AAA280268

• Gene Names: F2RL3; PAR4
• Reactivity: Human
• Synonyms: Proteinase-activated receptor 4; Human Proteinase-activated receptor 4 (F2RL3) ELISA Kit; F2RL3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human F2RL3. No significant cross-reactivity or interference between Human F2RL3 and analogues was observed.
• Sequence Length: 385

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for F2RL3 elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate F2RL3 in samples. An antibody specific for F2RL3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyF2RL3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for F2RL3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of F2RL3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 89353783
• NCBI GeneID: 9002
• NCBI Accession #: NP_003941.2
• NCBI GenBank Nucleotide #: NM_003950.3
• UniProt Accession #: Q96RI0; O76067; Q6DK42
• Molecular Weight: 41,133 Da
• NCBI Official Full Name: proteinase-activated receptor 4 preproprotein
• NCBI Official Synonym Full Names: F2R like thrombin or trypsin receptor 3
• NCBI Official Symbol: F2RL3
• NCBI Official Synonym Symbols: PAR4
• NCBI Protein Information: proteinase-activated receptor 4
• UniProt Protein Name: Proteinase-activated receptor 4
• Protein Family: Proteinase-activated receptor
• UniProt Gene Name: F2RL3
• UniProt Synonym Gene Names: PAR4; PAR-4

NCBI Description

This gene encodes a member of the protease-activated receptor subfamily, part of the G-protein coupled receptor 1 family of proteins. The encoded receptor is proteolytically processed to reveal an extracellular N-terminal tethered ligand that binds to and activates the receptor. This receptor plays a role in blood coagulation, inflammation and response to pain. Hypomethylation at this gene may be associated with lung cancer in human patients. [provided by RefSeq, Sep 2016]

Uniprot Description

PAR4: a G-protein coupled receptor for activated thrombin or trypsin. Coupled to G proteins that stimulate phosphoinositide hydrolysis. Plays a role in platelets activation. Thrombin requires PAR4 for full spreading on a fibrinogen matrix. Widely expressed, with highest levels in lung, pancreas, thyroid, testis and small intestine. Not expressed in brain, kidney, spinal cord and peripheral blood leukocytes. Also detected in platelets. A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand.

Protein type: GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 19p13.11

Cellular Component: extracellular region; integral component of plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; thrombin receptor activity

Biological Process: blood coagulation; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); phospholipase C-activating G-protein coupled receptor signaling pathway; platelet activation; platelet dense granule organization and biogenesis; positive regulation of release of sequestered calcium ion into cytosol; positive regulation of Rho protein signal transduction; response to wounding; signal transduction