PIVKA-II/Prothrombin Recombinant Protein | F2 recombinant protein

Recombinant Human PIVKA-II/Prothrombin protein, C-His Tag

Cat. Num. AAA1560048

• Gene Names: F2; PT; THPH1; RPRGL2
• Purity: >90% as determined by SDS-PAGE
• Synonyms: PIVKA-II/Prothrombin; Recombinant Human PIVKA-II/Prothrombin protein; C-His Tag; Coagulation factor II; F2 recombinant protein

• Host: Mammalian cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized. Lyophilized from PBS pH 7.4, 5% Trehalose, 5% Mannose, 1mM EDTA.

• Species: Human
• Reconstitution: Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details
• Construction: A DNA sequence encoding the human F2 (Met1-Glu622)with C-terminal His Tag
• Preparation and Storage: Store at 2 to 8 degree C for one week. Store at -20 to -80 degree C for twelve months from the date of receipt. Use a manual defrost freezer and avoid repeated freeze thaw cycles.; Shipping: In general, proteins are provided as lyophilized powder/frozen liquid.

SDS-Page

Product Categories/Family for F2 recombinant protein

Other proteins; Other proteins

NCBI and Uniprot Product Information

• NCBI GI #: 4503635
• NCBI GeneID: 2147
• NCBI Accession #: NP_000497.1
• NCBI GenBank Nucleotide #: NM_000506.3
• UniProt Accession #: P00734; Q4QZ40; Q53H04; Q53H06; Q7Z7P3; Q9UCA1; B2R7F7; B4E1A7
• Molecular Weight: 70,037 Da
• NCBI Official Full Name: prothrombin preproprotein
• NCBI Official Synonym Full Names: coagulation factor II (thrombin)
• NCBI Official Symbol: F2
• NCBI Official Synonym Symbols: PT; THPH1; RPRGL2
• NCBI Protein Information: prothrombin; serine protease; prothrombin B-chain; prepro-coagulation factor II
• UniProt Protein Name: Prothrombin
• Protein Family: Prothrombin
• UniProt Gene Name: F2
• UniProt Entry Name: THRB_HUMAN

NCBI Description

Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Mutations in F2 leads to various forms of thrombosis and dysprothrombinemia. [provided by RefSeq, Jul 2008]

Uniprot Description

prothrombin: Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. Defects in F2 are the cause of factor II deficiency (FA2D). It is a very rare blood coagulation disorder characterized by mucocutaneous bleeding symptoms. The severity of the bleeding manifestations correlates with blood factor II levels. Genetic variations in F2 may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Defects in F2 are the cause of thrombophilia due to thrombin defect (THPH1). It is a multifactorial disorder of hemostasis characterized by abnormal platelet aggregation in response to various agents and recurrent thrombi formation. A common genetic variation in the 3-prime untranslated region of the prothrombin gene is associated with elevated plasma prothrombin levels and an increased risk of venous thrombosis. Defects in F2 are associated with susceptibility to pregnancy loss, recurrent, type 2 (RPRGL2). A common complication of pregnancy, resulting in spontaneous abortion before the fetus has reached viability. The term includes all miscarriages from the time of conception until 24 weeks of gestation. Recurrent pregnancy loss is defined as 3 or more consecutive spontaneous abortions. Belongs to the peptidase S1 family.

Protein type: Secreted, signal peptide; EC 3.4.21.5; Protease; Apoptosis; Secreted

Chromosomal Location of Human Ortholog: 11p11

Cellular Component: extracellular space; endoplasmic reticulum lumen; Golgi lumen; extracellular region; plasma membrane

Molecular Function: protein binding; growth factor activity; serine-type endopeptidase activity; calcium ion binding; receptor binding

Biological Process: positive regulation of blood coagulation; multicellular organismal development; positive regulation of collagen biosynthetic process; proteolysis; regulation of cell shape; cell surface receptor linked signal transduction; negative regulation of fibrinolysis; positive regulation of cell proliferation; acute-phase response; response to wounding; negative regulation of proteolysis; platelet activation; cytosolic calcium ion homeostasis; post-translational protein modification; positive regulation of cell growth; peptidyl-glutamic acid carboxylation; positive regulation of phosphoinositide 3-kinase cascade; fibrinolysis; cellular protein metabolic process; regulation of gene expression; regulation of blood coagulation; negative regulation of astrocyte differentiation; positive regulation of release of sequestered calcium ion into cytosol; positive regulation of protein amino acid phosphorylation; blood coagulation; blood coagulation, intrinsic pathway; leukocyte migration

Disease: Thrombophilia Due To Thrombin Defect; Stroke, Ischemic; Prothrombin Deficiency, Congenital; Pregnancy Loss, Recurrent, Susceptibility To, 2