Nuclei Extraction Extraction Kit
Nuclei Extraction Kit (High Purity)
Synonyms
Nuclei Extraction; Nuclei Extraction Kit (High Purity); Nuclei Extraction extraction kit
Features & Benefits
Preparation of pure nuclei and fragile nuclei from mammalian cultured cells and solid tissues.
Protocol conducts ultracentrifugation through a dense sucrose cushion to protect nuclei and strip away membranes and cytoplasmic contaminants.
Protocol conducts ultracentrifugation through a dense sucrose cushion to protect nuclei and strip away membranes and cytoplasmic contaminants.
Usage Notes
Perform all steps at 2-8 degree C. Use precooled buffers and equipment. Ensure all the solutions are defrosted and homogeneous.
Preparation and Storage
Refer to list of materials supplied for storage conditions of individual components. Stable for at least 12 months at recommended temperature from date of shipment.
Related Product Information for Nuclei Extraction extraction kit
Description: Nuclei Extraction Kit (High Purity) is designed for the preparation of pure nuclei and fragile nuclei from mammalian cultured cells and solid tissues. Nuclei are collected by ultracentrifugation through a sucrose cushion and are quite clean and free of contaminating membranes and cytoplasmic components. The isolated nuclei can be preserved frozen several months in the included storage buffer.
Background: The preparation of an extract from nuclei is often the first step for many cell biology applications, such as in vitro apoptosis, examination of the transcriptional status of cells and a source of nuclear components (chromatin, genomic DNA, histones and nuclear RNA/RNP).
Background: The preparation of an extract from nuclei is often the first step for many cell biology applications, such as in vitro apoptosis, examination of the transcriptional status of cells and a source of nuclear components (chromatin, genomic DNA, histones and nuclear RNA/RNP).
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Product Notes
The Nuclei Extraction (Catalog #AAA9718636) is an Extraction Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Nuclei Extraction, Extraction Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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