Chicken Endothelin receptor B ELISA Kit | ETR-B elisa kit

Chicken Endothelin receptor B ELISA Kit

Cat. Num. AAA737596

• Gene Names: Ednrb; ETb; ET-B; ET-BR; ETR-b; Sox10m1
• Reactivity: Chicken
• Synonyms: Endothelin receptor B; Chicken Endothelin receptor B ELISA Kit; ETR-B elisa kit

• Reactivity: Chicken

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This ETR-B ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken ETR-B. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ETR-B elisa kit

Principle of the Assay: ETR-B ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ETR-B antibody and an ETR-B-HRP conjugate. The assay sample and buffer are incubated together with ETR-B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ETR-B concentration since ETR-B from samples and ETR-B-HRP conjugate compete for the anti-ETR-B antibody binding site. Since the number of sites is limited, as more sites are occupied by ETR-B from the sample, fewer sites are left to bind ETR-B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ETR-B concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ETR-B elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 443609487
• NCBI GeneID: 13618
• NCBI Accession #: NP_001263225.1
• NCBI GenBank Nucleotide #: NM_001276296.1
• UniProt Accession #: P48302; Q542M3
• Molecular Weight: 49,561 Da
• NCBI Official Full Name: endothelin B receptor
• NCBI Official Synonym Full Names: endothelin receptor type B
• NCBI Official Symbol: Ednrb
• NCBI Official Synonym Symbols: ETb; ET-B; ET-BR; ETR-b; Sox10m1
• NCBI Protein Information: endothelin B receptor; piebald; endothelin receptor non-selective type
• UniProt Protein Name: Endothelin B receptor
• UniProt Gene Name: Ednrb
• UniProt Synonym Gene Names: ET-B; ET-BR
• UniProt Entry Name: EDNRB_MOUSE

NCBI Description

This gene encodes a member of the G-protein coupled receptor family. It encodes a receptor for endothelins, peptides that are involved in vasocontriction. The encoded protein activates a phosphatidylinositol-calcium second messenger system and is required for the development of enteric neurons and melanocytes. Gene disruption causes pigmentation anomalies, deafness, and abnormal dilation of the colon due to defects of neural crest-derived cells. Mutations in this gene are found in the piebald mouse, and mouse models of Hirschsprung's disease and Waardenburg syndrome type 4. Renal collecting duct-specific gene deletion causes sodium retention and hypertension. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]

Uniprot Description

ETB: a G protein-coupled receptor which activates a phosphatidylinoitol-calcium second messenger system. Its ligand, endothelin, consists of a family of three potent vasoactive peptides: ET1, ET2, and ET3. Studies suggest that the multigenic disorder, Hirschsprung disease type 2, is due to mutation in endothelin receptor type B gene. Essential component in the normal development of two neuronal crest-derived cell lineages. Two splice variant isoforms have been described.

Protein type: Membrane protein, integral; GPCR, family 1; Membrane protein, multi-pass; Receptor, GPCR

Cellular Component: nuclear membrane; membrane; integral to membrane; plasma membrane; cytosol; lipid raft

Molecular Function: endothelin receptor activity; G-protein coupled receptor activity; signal transducer activity; protein binding; peptide hormone binding; type 1 angiotensin receptor binding

Biological Process: epithelial fluid transport; cGMP-mediated signaling; pigmentation during development; regulation of fever; response to pain; response to lipopolysaccharide; negative regulation of cellular protein metabolic process; negative regulation of transcription from RNA polymerase II promoter; vein smooth muscle contraction; signal transduction; sensory perception of pain; enteric nervous system development; vasodilation; regulation of epithelial cell proliferation; elevation of cytosolic calcium ion concentration; regulation of blood pressure; positive regulation of cell proliferation; melanocyte differentiation; regulation of pH; neural crest cell migration; posterior midgut development; vasoconstriction; macrophage chemotaxis; peripheral nervous system development; G-protein coupled receptor protein signaling pathway; negative regulation of neuron maturation; pigmentation; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); regulation of sensory perception of pain; positive regulation of protein amino acid phosphorylation; negative regulation of apoptosis