Insulin Like Growth Factor Binding Protein-3 Active Protein | IGFBP3 active protein

Recombinant Human Insulin Like Growth Factor Binding Protein-3

Cat. Num. AAA142177

• Gene Names: IGFBP3; IBP3; BP-53
• Purity: Greater than 97.0% as determined by: ; (a) Analysis by RP-HPLC.; (b) Analysis by SDS-PAGE.
• Synonyms: Insulin Like Growth Factor Binding Protein-3; Recombinant Human Insulin Like Growth Factor Binding Protein-3; IGFBP 3 Human; Insulin-Like Growth Factor Binding Protein-3 Human Recombinant; Growth-hormone-dependant binding protein; IBP3; BP-53; IGFBP-3; IGFBP3 active protein

• Host: E Coli
• Purity/Purification: Greater than 97.0% as determined by: ; (a) Analysis by RP-HPLC.; (b) Analysis by SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 um filtered concentrated (0.5mg/ml) solution in PBS, pH 7.4.
• Sequence: GASSGGLGPV VRCEPCDARA LAQCAPPPAV CAELVREPGC GCCLTCALSE GQPCGIYTER CGSGLRCQPS PDEARPLQAL LDGRGLCVNA SAVSRLRAYL LPAPPAPGNA SESEEDRSAG EVESPSVSST HRVSDPKFHP LHSKIIIIKK GHAKDSQRYK VDYESQSTDT QNFSSESKRE TEYGPCRREM EDTLNHLKFL NVLSPRGVHI PNCDKKGFYK KKQCRPSKGR KRGFCWCVDK YGQPLPGYTT KGKEDVHCYS MQSK
• Sequence Length: 291

• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Solubility: It is recommended to reconstitute the lyophilized Insulin-Like Growth Factor Binding Protein-3 in sterile 20mM AcOH (acetic Acid) not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: The ED₅₀ calculated by its ability to inhibit IGF-II induced proliferation of MCF-7 is < 0.2 ug/ml in the presence of 15 ng/ml of Human IGF-II, corresponding to specific activity of 5,000,000 units/mg.
• Preparation and Storage: Lyophilized IBP3 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution IGF-BP 3 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Related Product Information for IGFBP3 active protein

IGFBP3 Human Recombinant produced in E Coli is a homodimeric, non-glycosylated, polypeptide chain containing 2x264 amino acids and having a molecular mass of 28806 Dalton. IGFBP-3 is purified by proprietary chromatographic techniques.

Product Categories/Family for IGFBP3 active protein

Cytokines and Growth Factors; Growth Factors; IGFBP

NCBI and Uniprot Product Information

• NCBI GI #: 62243068
• NCBI GeneID: 3486
• NCBI Accession #: NP_000589.2
• NCBI GenBank Nucleotide #: NM_000598.4
• UniProt Accession #: P17936; Q2V509; Q6P1M6; Q9UCL4; A4D2F5; D3DVM0
• NCBI Official Full Name: insulin-like growth factor-binding protein 3 isoform b
• NCBI Official Synonym Full Names: insulin-like growth factor binding protein 3
• NCBI Official Symbol: IGFBP3
• NCBI Official Synonym Symbols: IBP3; BP-53
• NCBI Protein Information: insulin-like growth factor-binding protein 3; IBP-3; IGF-binding protein 3; IGFBP-3; acid stable subunit of the 140 K IGF complex; binding protein 29; binding protein 53; growth hormone-dependent binding protein
• UniProt Protein Name: Insulin-like growth factor-binding protein 3
• Protein Family: Insulin-like growth factor-binding protein
• UniProt Gene Name: IGFBP3
• UniProt Synonym Gene Names: IBP3; IBP-3; IGF-binding protein 3; IGFBP-3
• UniProt Entry Name: IBP3_HUMAN

NCBI Description

This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

IGFBP3: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R.

Protein type: Cell development/differentiation; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7p12.3

Cellular Component: insulin-like growth factor binding protein complex; extracellular space; extracellular region; nucleus

Molecular Function: insulin-like growth factor binding; protein binding; insulin-like growth factor I binding; metal ion binding; fibronectin binding; insulin-like growth factor II binding; protein tyrosine phosphatase activator activity

Biological Process: positive regulation of insulin-like growth factor receptor signaling pathway; positive regulation of catalytic activity; regulation of insulin-like growth factor receptor signaling pathway; negative regulation of smooth muscle cell proliferation; apoptosis; positive regulation of apoptosis; negative regulation of smooth muscle cell migration; protein amino acid phosphorylation; positive regulation of myoblast differentiation; negative regulation of signal transduction; osteoblast differentiation; negative regulation of cell proliferation; cellular protein metabolic process; negative regulation of protein amino acid phosphorylation; positive regulation of MAPKKK cascade; regulation of cell growth