Pegylated leptin antagonist Active Protein | LEP active protein

Pegylated mouse leptin antagonist (mutant L39A/D40A/F41A)

Cat. Num. AAA400045

• Gene Names: LEP; OB; OBS; LEPD
• Purity: The purity of recombinant mouse pegylated leptin antagonist is more than 98.0% as determined by:; (a) Gel filtration analysis.; (b) Analysis by reducing and non-reducing SDS-PAGE gel.
• Synonyms: Pegylated leptin antagonist; Pegylated mouse leptin antagonist (mutant L39A/D40A/F41A); Mouse pegylated leptin antagonist (mutant L39A/D40A/F41A); LEP active protein

• Host: Escherichia Coli
• Purity/Purification: The purity of recombinant mouse pegylated leptin antagonist is more than 98.0% as determined by:; (a) Gel filtration analysis.; (b) Analysis by reducing and non-reducing SDS-PAGE gel.
• Form/Format: The recombinant mouse pegylated leptin antagonist was lyophilized from a concentrated (0.65mg/ml) solution with 0.003mM NaHCO3.
• Sequence: The sequence of the N-terminal amino acids of recombinant mouse pegylated leptin antagonist could not be determined indicating that the PEG moiety is attached to the N-terminus.
• Sequence Length: 167

• Solubility: The recombinant mouse pegylated leptin antagonist was lyophilized from a concentrated (0.65mg/ml) solution with 0.003mM NaHCO3. It is recommended to reconstitute the lyophilized recombinant mouse pegylated leptin antagonist in sterile water or sterile 0.4% NaHCO3 adjusted to pH 8-9, not less than 100ug/ml, which can then be further diluted with other aqueous solutions.
• Protein Content: Protein quantization of recombinant mouse pegylated leptin antagonist was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.201 as the extinction coefficient for a 0.1% (1mg/ml) protein solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
• Dimers and Aggregates: The purified recombinant mouse pegylated leptin antagonist consists of > 90% monomers as determined by gel-filtration chromatography.
• Appearance: White lyophilized (freeze-dried) powder.
• Endotoxin: The endotoxin content of pegylated recombinant mouse leptin antagonist is less than 0.05 ng/ug (IEU/ug).
• Formulation: The recombinant mouse pegylated leptin antagonist was lyophilized from a concentrated (0.65mg/ml) solution with 0.003mM NaHCO3.
• Biological Activity: Recombinant mouse pegylated leptin antagonist is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. The in vitro activity of the pegylated recombinant mouse leptin antagonistis 6-8 fold lower than the non-pegylated antagonist but in vivo it has profound weight gain effect (as compared to the non-pegylated recombinant mouse leptin antagonist), resulting mainly from increased food intake.
• Preparation and Storage: Lyophilized recombinant mouse pegylated leptin antagonist although stable at room temperature for several weeks, should be stored desiccated below -18°C. Upon reconstitution at > 0.1 recombinant mouse pegylated leptin antagonist mg/ml and up to 2 mg/ml and filter sterilization recombinant mouse pegylated leptin antagonist can be stored at 4°C or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration of recombinant mouse pegylated leptin antagonist addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles.

Related Product Information for LEP active protein

Mono-pegylated (with 20 kDa PEG) recombinant mouse leptin antagonist is one polypeptide chain containing 146 amino, an additional Ala at N-terminus and one molecule of PEG 20 kDa at its N-terminus, having an expected molecular mass of ~ 35.6 kDa as determined by MS. However due to enlarged hydrodymanic volume mouse pegylated leptin antagonist runs on the SGS-Page as 48 kDa protein and in gel-filtration on Superdex 200 as over 200 kDa protein. Mouse pegylated leptin antagonist half-life in circulation after SC injection was over 20 hours.. Mouse leptin antagonist was initially mutated, resulting in L39A/D40A/F41A mutant that was purified by proprietary chromatographic techniques according to Salomon et al (2006) Protein Expression and Purification 47, 128 -136 and its pegylation is described in Elinav et al. Endocrinology 150:3083-91 (2009).

Product Categories/Family for LEP active protein

Leptin and leptin muteins

NCBI and Uniprot Product Information

• NCBI GI #: 4557715
• NCBI GeneID: 3952
• NCBI Accession #: NP_000221.1
• NCBI GenBank Nucleotide #: NM_000230.2
• UniProt Accession #: P41159; O15158; Q56A88
• NCBI Official Full Name: leptin
• NCBI Official Synonym Full Names: leptin
• NCBI Official Symbol: LEP
• NCBI Official Synonym Symbols: OB; OBS; LEPD
• NCBI Protein Information: leptin; leptin (murine obesity homolog); leptin (obesity homolog, mouse); obese protein; obese, mouse, homolog of; obesity factor
• UniProt Protein Name: Leptin
• Protein Family: Leptin
• UniProt Gene Name: LEP
• UniProt Synonym Gene Names: OB; OBS
• UniProt Entry Name: LEP_HUMAN

NCBI Description

This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq, Jul 2008]

Uniprot Description

leptin: May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. Defects in LEP may be a cause of obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Belongs to the leptin family.

Protein type: Secreted; Cell development/differentiation; Secreted, signal peptide; Hormone

Chromosomal Location of Human Ortholog: 7q31.3

Cellular Component: extracellular space; cytoplasm; extracellular region

Molecular Function: growth factor activity; peptide hormone receptor binding; hormone activity

Biological Process: circadian rhythm; response to dietary excess; positive regulation of myeloid cell differentiation; regulation of fat cell differentiation; regulation of steroid biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; female pregnancy; glucose homeostasis; negative regulation of appetite; positive regulation of luteinizing hormone secretion; response to insulin stimulus; positive regulation of tyrosine phosphorylation of Stat3 protein; response to vitamin E; regulation of cholesterol absorption; positive regulation of MAPKKK cascade; regulation of blood pressure; positive regulation of cell proliferation; positive regulation of ion transport; central nervous system neuron development; placenta development; positive regulation of cytokine production; cholesterol metabolic process; positive regulation of developmental growth; bile acid metabolic process; eating behavior; glucose metabolic process; adult feeding behavior; ovulation from ovarian follicle; leptin-mediated signaling pathway; negative regulation of vasoconstriction; tyrosine phosphorylation of STAT protein; fatty acid beta-oxidation; glycerol biosynthetic process; insulin secretion; response to hypoxia; energy reserve metabolic process; hormone metabolic process; regulation of gluconeogenesis; positive regulation of insulin receptor signaling pathway; positive regulation of follicle-stimulating hormone secretion; leukocyte tethering or rolling; regulation of insulin secretion; negative regulation of apoptosis

Disease: Leptin Deficiency