interferon alpha 2A Active Protein
Human interferon alpha 2A
Purity
Greater than 98.0% as determined by both:(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Synonyms
interferon alpha 2A; Human interferon alpha 2A; Human interferon-alpha 2A; interferon alpha 2A active protein
Host
Escherichia Coli
Purity/Purification
Greater than 98.0% as determined by both:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Form/Format
Sterile Filtered White lyophilized (freeze-dried) powder.
Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Cys-Asp-Leu-Pro-Gln, conforming to the sequence of native human IFN-alpha. The N-terminal methionine has been completely removed enzymatically.
Solubility
It is recommended to reconstitute the lyophilized Interferon-alpha 2A in sterile 18Momega-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Protein Content
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.924 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of IFN-alpha 2A as a Reference Standard.
1. UV spectroscopy at 280 nm using the absorbency value of 0.924 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of IFN-alpha 2A as a Reference Standard.
Dimers and Aggregates
Over 95% monomers
Endotoxin
Less than 0.1 ng/ug (IEU/ug) of INF-alpha 2A
Formulation
Lyophilized from (1mg/ml) solution containing 7.21 mg sodium chloride and 0.77 mg ammonium acetate per ml.
Biological Activity
The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 2.7 x 10E8 IU/mg.
Preparation and Storage
Lyophilized Interferon alpha 2A although stable sterile at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution IFN-alpha 2A should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Related Product Information for interferon alpha 2A active protein
IFN-alpha is produced by macrophages and has antiviral activities. Interferon stimulates the production of two enzymes: protein kinase and an oligoadenylate synthetase. Recombinant human interferon alpha 2A is produced in E.Coli as a single, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 19241 Dalton according to the sequence of interferon-alpha 2A gene obtained from human leukocytes. The IFN-alpha 2A was purified by proprietary chromatographic techniques.
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Product Notes
The interferon alpha 2A (Catalog #AAA400094) is an Active Protein produced from Escherichia Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: The sequence of the first five N-terminal amino acids was determined and was found to be Cys-Asp-Le u-Pro-Gln, conforming to the sequence of native human IFN-alpha. The N-terminal methionine has been completely removed enzymatica lly. It is sometimes possible for the material contained within the vial of "interferon alpha 2A, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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