Typical Testing Data/Standard Curve (for reference only)
Rat E-selectin ELISA Kit | ES elisa kit
Rat E-selectin ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ES enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific forES. Standards or samples are then added to the microtiter plate wells andES if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount ofES present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific forES are added to each well to "sandwich" theES immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that containES and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is found in cytokine-stimulated endothelial cells and is thought to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. It exhibits structural features such as the presence of lectin- and EGF-like domains followed by short consensus repeat (SCR) domains that contain 6 conserved cysteine residues. These proteins are part of the selectin family of cell adhesion molecules. Adhesion molecules participate in the interaction between leukocytes and the endothelium and appear to be involved in the pathogenesis of atherosclerosis. [provided by RefSeq]
Uniprot Description
SELE: Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis. Belongs to the selectin/LECAM family.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 1q22-q25
Cellular Component: cortical cytoskeleton; extracellular space; perinuclear region of cytoplasm; integral to membrane; plasma membrane; coated pit; caveola; lipid raft
Molecular Function: protein binding; transmembrane receptor activity; phospholipase binding; sialic acid binding
Biological Process: heterophilic cell adhesion; leukocyte adhesion; phospholipase C activation; positive regulation of leukocyte migration; positive regulation of receptor internalization; regulation of inflammatory response; actin filament-based process; calcium-mediated signaling; response to lipopolysaccharide; blood coagulation; leukocyte tethering or rolling; inflammatory response; leukocyte migration; leukocyte migration during inflammatory response
Disease: Hypertension, Essential
Research Articles on ES
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Product Notes
The Rat ES sele (Catalog #AAA731559) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA731559 ELISA Kit recognizes Rat ES. It is sometimes possible for the material contained within the vial of "E-selectin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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