ERP44 recombinant protein

Recombinant Human ERP44 Protein

Cat. Num. AAA8248992

• Gene Names: ERP44; PDIA10; TXNDC4
• Reactivity: Human
• Applications: ELISA, Western Blot, SDS-Page
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Synonyms: ERP44; Recombinant Human ERP44 Protein; KIAA0573; TXNDC4; Endoplasmic reticulum resident protein 44; ER protein 44; ERp44; Thioredoxin domain-containing protein 4; ERP44 recombinant protein

• Host: Human cells
• Reactivity: Human
• Purity/Purification: Greater than 95% as determined by reducing SDS-PAGE.
• Form/Format: Supplied as a 0.2 muM filtered solution of 20mM Tris-HCl, 10% Glycerol, pH 7.5.
• Sequence: EITSLDTENI DEILNNADVA LVNFYADWCR FSQMLHPIFE EASDVIKEEF PNENQVVFAR VDCDQHSDIA QRYRISKYPT LKLFRNGMMM KREYRGQRSV KALADYIRQQ KSDPIQEIRD LAEITTLDRS KRNIIGYFEQ KDSDNYRVFE RVANILHDDC AFLSAFGDVS KPERYSGDNI IYKPPGHSAP DMVYLGAMTN FDVTYNWIQD KCVPLVREIT FENGEELTEE GLPFLILFHM KEDTESLEIF QNEVARQLIS EKGTINFLHA DCDKFRHPLL HIQKTPADCP VIAIDSFRHM YVFGDFKDVL IPGKLKQFVF DLHSGKLHRE FHHGPDPTDT APGEQAQDVA SSPPESSFQK LAPSEYRYTL LRDVDHHHHH H
• Sequence Length: 406

• Applicable Applications for ERP44 recombinant protein: ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
• Endotoxin: Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
• Directions for Use: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Preparation and Storage: Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.

Related Product Information for ERP44 recombinant protein

Recombinant Human ERP44 Protein is produced by our mammalian expression system and the target gene encoding Glu30-Asp402 is expressed with a 6His tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 52487191
• NCBI GeneID: 23071
• NCBI Accession #: NP_055866.1
• NCBI GenBank Nucleotide #: NM_015051.2
• UniProt Accession #: Q9BS26; O60319; Q4VXC1; Q5VWZ7; Q6UW14; Q8WX67
• Molecular Weight: 46,971 Da
• NCBI Official Full Name: endoplasmic reticulum resident protein 44
• NCBI Official Synonym Full Names: endoplasmic reticulum protein 44
• NCBI Official Symbol: ERP44
• NCBI Official Synonym Symbols: PDIA10; TXNDC4
• NCBI Protein Information: endoplasmic reticulum resident protein 44
• UniProt Protein Name: Endoplasmic reticulum resident protein 44
• Protein Family: Endoplasmic reticulum resident protein
• UniProt Gene Name: ERP44
• UniProt Synonym Gene Names: KIAA0573; TXNDC4; ER protein 44; ERp44
• UniProt Entry Name: ERP44_HUMAN

NCBI Description

This gene encodes a member of the protein disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins. It has an inferred N-terminal signal peptide, a catalytically active thioredoxin (TRX) domain, two TRX-like domains and a C-terminal ER-retention sequence. This protein functions as a pH-regulated chaperone of the secretory pathway and likely plays a role in protein quality control at the endoplasmic reticulum - Golgi interface. [provided by RefSeq, Dec 2016]

Uniprot Description

ERP44: Mediates thiol-dependent retention in the early secretory pathway, forming mixed disulfides with substrate proteins through its conserved CRFS motif. Inhibits the calcium channel activity of ITPR1. May have a role in the control of oxidative protein folding in the endoplasmic reticulum. Required to retain ERO1L and ERO1LB in the endoplasmic reticulum. Forms mixed disulfides with both ERO1L and ERO1LB and cargo folding intermediates. Directly interacts with ITPR1 in a pH-, redox state- and calcium-dependent manner, but not with ITPR2 or ITPR3. The strength of this interaction inversely correlates with calcium concentration. By endoplasmic reticulum stress.

Protein type: Endoplasmic reticulum; Isomerase; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9q31.1

Cellular Component: cell surface; endoplasmic reticulum lumen; endoplasmic reticulum membrane; ER-Golgi intermediate compartment

Molecular Function: protein binding; protein disulfide isomerase activity

Biological Process: cell redox homeostasis; glycoprotein metabolic process; protein folding; response to unfolded protein