ERN1 / Serine/Threonine-Protein Kinase/Endoribonuclease IRE1 Recombinant Protein | ERN1 recombinant protein
Human ERN1 / Serine/Threonine-Protein Kinase/Endoribonuclease IRE1 Recombinant Protein
Gene Names
ERN1; IRE1; IRE1P; IRE1a; hIRE1p
Applications
ELISA, Western Blot
Purity
>95% by SDS-PAGE
Synonyms
ERN1 / Serine/Threonine-Protein Kinase/Endoribonuclease IRE1; Human ERN1 / Serine/Threonine-Protein Kinase/Endoribonuclease IRE1 Recombinant Protein; Serine/threonine-protein kinase/endoribonuclease IRE1; Endoplasmic reticulum-to-nucleus signaling 1; Inositol-requiring protein 1; hIRE1p; Ire1-alpha; IRE1a; IRE1; ERN1 recombinant protein
Host
E Coli
Purity/Purification
>95% by SDS-PAGE
Form/Format
Liquid; 50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10% Glycerol (PH8.0)
Concentration
Reconstitution Dependent (varies by lot)
Sequence Length
977
Applicable Applications for ERN1 recombinant protein
ELISA (EIA), Western Blot (WB), Antigen Protein (AP), Protein Array
Species
Human
Tag
His
Subunit
Interacts with DAB2IP (via PH domain); the interaction occurs in a ER stress-induced dependent manner and is required for subsequent recruitment of TRAF2 to ERN1 (By similarity). Homodimer; disulfide-linked. Dimer formation is driven by hydrophobic interactions within the N-terminal luminal domains and stabilized by disulfide bridges. Also binds HSPA5, a negative regulator of the unfolded protein response. This interaction may disrupt homodimerization and prevent activation of ERN1. Interacts with TAOK3 and TRAF2.
Entry Function
Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.
Preparation and Storage
Store at -20 degree C. Avoid repeated freezing and thawing.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Serine/threonine-protein kinase/endoribonuclease IRE1
NCBI Official Synonym Full Names
endoplasmic reticulum to nucleus signaling 1
NCBI Official Symbol
ERN1
NCBI Official Synonym Symbols
IRE1; IRE1P; IRE1a; hIRE1p
NCBI Protein Information
serine/threonine-protein kinase/endoribonuclease IRE1
UniProt Protein Name
Serine/threonine-protein kinase/endoribonuclease IRE1
UniProt Gene Name
ERN1
UniProt Synonym Gene Names
hIRE1p; IRE1a
UniProt Entry Name
ERN1_HUMAN
NCBI Description
This gene encodes the transmembrane protein kinase inositol-requiring enzyme 1. The encoded protein contains two functional catalytic domains, a serine/threonine-protein kinase domain and an endoribonuclease domain. This protein functions as a sensor of unfolded proteins in the endoplasmic reticulum (ER) and triggers an intracellular signaling pathway termed the unfolded protein response (UPR). The UPR is an ER stress response that is conserved from yeast to mammals and activates genes involved in degrading misfolded proteins, regulating protein synthesis and activating molecular chaperones. This protein specifically mediates the splicing and activation of the stress response transcription factor X-box binding protein 1. [provided by RefSeq, Aug 2017]
Research Articles on ERN1
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Product Notes
The ERN1 ern1 (Catalog #AAA2903499) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's ERN1 / Serine/Threonine-Protein Kinase/Endoribonuclease IRE1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Antigen Protein (AP), Protein Array. Researchers should empirically determine the suitability of the ERN1 ern1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ERN1 / Serine/Threonine-Protein Kinase/Endoribonuclease IRE1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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