Typical Testing Data/Standard Curve (for reference only)
Rat Estrogen Receptor Beta ELISA Kit | ERbeta elisa kit
Rat Estrogen Receptor Beta ELISA Kit
Gene Names
ESR2; ERB; ERBETA
Reactivity
Rat
Synonyms
Estrogen Receptor Beta; Rat Estrogen Receptor Beta ELISA Kit; ERbeta elisa kit
Reactivity
Rat
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
0.1 ng/mL.
Intended Uses
This ERbeta ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat ERbeta. This ELISA kit for research use only, not for therapeutic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for ERbeta elisa kit
Principle of the assay: ERbeta ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ERbeta antibody and an ERbeta-HRP conjugate. The assay sample and buffer are incubated together with ERbeta-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ERbeta concentration since ERbeta from samples and ERbeta-HRP conjugate compete for the anti-ERbeta antibody binding site. Since the number of sites is limited, as more sites are occupied by ERbeta from the sample, fewer sites are left to bind ERbeta-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ERbeta concentration in each sample is interpolated from this standard curve.
Product Categories/Family for ERbeta elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36,176 Da
NCBI Official Full Name
estrogen receptor beta
NCBI Official Symbol
ESR2
NCBI Official Synonym Symbols
ERB; ERBETA
NCBI Protein Information
estrogen receptor beta; ER-beta; steroid receptor; nuclear receptor subfamily 3 group A member 2
UniProt Protein Name
Estrogen receptor beta
Protein Family
UniProt Gene Name
ESR2
UniProt Synonym Gene Names
NR3A2; ER-beta
UniProt Entry Name
ESR2_SHEEP
Uniprot Description
Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner.
Research Articles on ERbeta
Similar Products
Product Notes
The Rat ERbeta esr2 (Catalog #AAA727959) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA727959 ELISA Kit recognizes Rat ERbeta. It is sometimes possible for the material contained within the vial of "Estrogen Receptor Beta, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
