Epidermal Growth Factor Receptor 2 (EGFR2) Recombinant Protein | EGFR2 recombinant protein

Recombinant Epidermal Growth Factor Receptor 2 (EGFR2)

Cat. Num. AAA2011286

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Epidermal Growth Factor Receptor 2 (EGFR2); Recombinant Epidermal Growth Factor Receptor 2 (EGFR2); EGFR2 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-F LPESFDGDPS SGIAPLRPEQ LQVFETLEEI TGYLYISAWP DSLRDLSVFQ NLRIIRGRIL HDGAYSLTLQ GLGIHSLGLR SLRELGSGLA LIHRNAHLCF VHTVPWDQLF RNPHQALLHS GNRPEEDCGL EGLVCNSLCA HGHCWGPGPT QCVNCSHFLR GQECVEECRV WKGLPREYVS DKRCLPCHPE CQPQNSSETC FG
• Sequence Length: 1259

• Applicable Applications for EGFR2 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Phe380-Gly582 (Accession # Q8K3F9) with two N-terminal Tags, His-tag and GST-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 77020289
• NCBI GeneID: 24337
• NCBI Accession #: NP_058699.2
• NCBI GenBank Nucleotide #: NM_017003.2
• UniProt Accession #: Q8K3F9; P06494
• Molecular Weight: 50.0kDa
• NCBI Official Full Name: receptor tyrosine-protein kinase erbB-2
• NCBI Official Synonym Full Names: v-erb-b2 avian erythroblastic leukemia viral oncogene 2
• NCBI Official Symbol: Erbb2
• NCBI Protein Information: receptor tyrosine-protein kinase erbB-2; p185neu; C-erbB-2; p185erbB2; NEU proto-oncogene; proto-oncogene NEU; proto-oncogene c-ErbB-2; epidermal growth factor receptor-related protein; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog; Avian erythroblastosis viral (v-erb-B2) oncogene homologue 2 (neuro/glioblastoma derived oncogene homolog)
• UniProt Protein Name: Neu protooncoprotein
• UniProt Gene Name: Erbb2
• UniProt Entry Name: Q8K3F9_RAT

NCBI Description

may play a role in cell proliferation and differentiation [RGD, Feb 2006]

Uniprot Description

HER2: a proto-oncogenic receptor tyrosine kinase of the EGFR family. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. Not activated by EGF, TGF- alpha and amphiregulin. Amplified in breast cancer. Overexpression induces constitutive activity, and the gene is amplified or overexpressed in up to 30% of breast cancers, correlating with poor survival. The antibody Herceptin is approved for treatment of metastatic breast cancer with HER2 amplification/overexpression. Somatic mutations seen in 4% of lung cancers and also in breast, gastric, ovarian cancer and glioblastoma. One SNP shows predisposition to breast and gastric cancer. Inhibitors: Herceptin, lapatinib, PKI-166, EKB-569, CI-1033.

Protein type: Membrane protein, integral; Protein kinase, tyrosine (receptor); Protein kinase, TK; Oncoprotein; EC 2.7.10.1; Kinase, protein; TK group; EGFR family

Cellular Component: microvillus; basolateral plasma membrane; lateral loop; integral to membrane; lipid raft; postsynaptic membrane; perinuclear region of cytoplasm; cytoplasm; apical plasma membrane; basal plasma membrane; plasma membrane; endosome membrane; cytoplasmic vesicle; nucleus; receptor complex

Molecular Function: protein C-terminus binding; identical protein binding; Hsp90 protein binding; transmembrane receptor protein tyrosine kinase activity; receptor signaling protein tyrosine kinase activity; protein phosphatase binding; protein binding; transmembrane receptor activity; DNA binding; protein heterodimerization activity; growth factor binding; protein-tyrosine kinase activity; ubiquitin protein ligase binding; glycoprotein binding; ATP binding

Biological Process: myelination; tongue development; mammary gland involution; phosphoinositide 3-kinase cascade; positive regulation of cell adhesion; central nervous system development; peptidyl-tyrosine phosphorylation; wound healing; positive regulation of translation; protein amino acid autophosphorylation; heart development; motor axon guidance; signal transduction; positive regulation of transcription from RNA polymerase I promoter; positive regulation of MAP kinase activity; sympathetic nervous system development; glial cell differentiation; cell surface receptor linked signal transduction; positive regulation of MAPKKK cascade; regulation of cell differentiation; positive regulation of cell proliferation; oligodendrocyte differentiation; response to axon injury; neuromuscular junction development; response to drug; negative regulation of immature T cell proliferation in the thymus; nervous system development; regulation of microtubule-based process; skeletal muscle development; transcription, DNA-dependent; liver development; positive regulation of cell growth; regulation of cell proliferation; peripheral nervous system development; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of transcription from RNA polymerase III promoter; positive regulation of protein amino acid phosphorylation; positive regulation of Ras protein signal transduction; response to progesterone stimulus; positive regulation of epithelial cell proliferation; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis