ERBB2 recombinant protein

Recombinant Rat ERBB2 Protein

Cat. Num. AAA8248987

• Reactivity: Rat
• Applications: ELISA, Western Blot, SDS-Page
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Synonyms: ERBB2; Recombinant Rat ERBB2 Protein; HER2; MLN19; NEU; NGL; Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; MLN 19; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; CD340; ERBB2 recombinant protein

• Host: E Coli
• Reactivity: Rat
• Purity/Purification: Greater than 95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 muM filtered solution of 20mM Tris, 150mM NaCl, 4M Urea, pH 8.0.
• Sequence: MANASLSFLQ DIQEVQGYML IAHNQVKRVP LQRLRIVRGT QLFEDKYALA VLDNRDPQDN VAASTPGRTP EGLRELQLRS LTEILKGGVL IRGNPQLCYQ DMVLWKDVFR KNNQLAPVDI DTNRSRACPP CAPACKDNHC WGESPEDCQI LTGTICTSGC ARCKGRLPTD CCHEQCAAGC TGPKHSDCLA CLHFNHSGIC ELHCPALVTY NTDTFESMHN PEGRYTFGAS CVTTCPYNYL STEVGSCTLV CPPNNQEVHH HHHH
• Sequence Length: 1257

• Applicable Applications for ERBB2 recombinant protein: ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
• Endotoxin: Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
• Directions for Use: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Preparation and Storage: Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.

Related Product Information for ERBB2 recombinant protein

Recombinant Rat ERBB2 Protein is produced by our E Coli expression system and the target gene encoding Ala67-Val323 is expressed with a 6His tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 3915663
• NCBI GeneID: 24337
• UniProt Accession #: P06494; Q6P732
• Molecular Weight: 138,832 Da
• NCBI Official Full Name: Receptor tyrosine-protein kinase erbB-2
• NCBI Official Synonym Full Names: erb-b2 receptor tyrosine kinase 2
• NCBI Official Symbol: Erbb2
• NCBI Protein Information: receptor tyrosine-protein kinase erbB-2
• UniProt Protein Name: Receptor tyrosine-protein kinase erbB-2
• Protein Family: Receptor tyrosine-protein kinase
• UniProt Gene Name: Erbb2
• UniProt Synonym Gene Names: Neu
• UniProt Entry Name: ERBB2_RAT

NCBI Description

may play a role in cell proliferation and differentiation [RGD, Feb 2006]

Uniprot Description

HER2: a proto-oncogenic receptor tyrosine kinase of the EGFR family. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. Not activated by EGF, TGF- alpha and amphiregulin. Amplified in breast cancer. Overexpression induces constitutive activity, and the gene is amplified or overexpressed in up to 30% of breast cancers, correlating with poor survival. The antibody Herceptin is approved for treatment of metastatic breast cancer with HER2 amplification/overexpression. Somatic mutations seen in 4% of lung cancers and also in breast, gastric, ovarian cancer and glioblastoma. One SNP shows predisposition to breast and gastric cancer. Inhibitors: Herceptin, lapatinib, PKI-166, EKB-569, CI-1033.

Protein type: EC 2.7.10.1; EGFR family; Kinase, protein; Membrane protein, integral; Oncoprotein; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group

Cellular Component: apical plasma membrane; basal plasma membrane; basolateral plasma membrane; cytoplasm; cytoplasmic vesicle; endosome membrane; lateral loop; lipid raft; microvillus; myelin sheath; nucleus; perinuclear region of cytoplasm; plasma membrane; postsynaptic membrane; receptor complex

Molecular Function: glycoprotein binding; growth factor binding; Hsp90 protein binding; identical protein binding; protein binding; protein C-terminus binding; protein heterodimerization activity; protein phosphatase binding; protein-tyrosine kinase activity; transmembrane receptor activity; transmembrane receptor protein tyrosine kinase activity; ubiquitin protein ligase binding

Biological Process: cell surface receptor linked signal transduction; central nervous system development; glial cell differentiation; heart development; liver development; mammary gland involution; motor axon guidance; myelination; negative regulation of apoptosis; negative regulation of immature T cell proliferation in the thymus; nervous system development; neuromuscular junction development; oligodendrocyte differentiation; peripheral nervous system development; phosphoinositide 3-kinase cascade; positive regulation of cell adhesion; positive regulation of cell growth; positive regulation of cell proliferation; positive regulation of epithelial cell proliferation; positive regulation of GTPase activity; positive regulation of MAP kinase activity; positive regulation of MAPKKK cascade; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein amino acid phosphorylation; positive regulation of Ras protein signal transduction; positive regulation of transcription from RNA polymerase I promoter; positive regulation of transcription from RNA polymerase III promoter; positive regulation of translation; protein amino acid autophosphorylation; regulation of cell differentiation; regulation of cell proliferation; regulation of microtubule-based process; response to axon injury; response to drug; response to progesterone stimulus; signal transduction; skeletal muscle development; sympathetic nervous system development; tongue development; transmembrane receptor protein tyrosine kinase signaling pathway; wound healing