BDNF elisa kit

Horse equine BDNF PicoKine ELISA Kit

Cat. Num. AAA1751011

• Gene Names: BDNF; ANON2; BULN2
• Reactivity: Equine; There is no detectable cross-reactivity with other relevant proteins.
• Applications: ELISA
• Synonyms: BDNF; Horse equine BDNF PicoKine ELISA Kit; Brain-derived neurotrophic factor; Abrineurin; BDNF elisa kit

• Reactivity: Equine; There is no detectable cross-reactivity with other relevant proteins.
• Specificity: Natural and recombinant 216 Horse equine BDNF
• Sequence Length: 276

• Applicable Applications for BDNF elisa kit: ELISA (EIA)
• Assay Type: Quantitative Sandwich
• Detection Range: 31.2pg/ml-2000pg/ml
• Sensitivity: <15pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration weretested on one plate to assess intra-assay precision
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration weretested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BDNF elisa kit

Principle of the Assay: 216 Horse equine BDNF ELISA Kit was based on standard sandwich enzyme-linked immune- sorbent assay technology. A monoclonal antibody from mouse specific for Horse equine BDNF has been precoated onto 96-well plates. Standards(Expression system for standard: sf21; Immunogen sequence: H129-R247) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Horse equine BDNF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the 216 Horse equine BDNF amount of sample captured in plate.

Background: Brain-derived neurotrophic factor(BDNF) is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is a secreted protein with the molecular weight of 27.8kDa, consisting of 247 amino acids. It is known to promote neuronal survival and differentiation. BDNF shares substantial amino acid sequence identity with nerve growth factor(NGF). BDNF and neurotrophin-3(NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult horse equine brain, suggesting that these proteins are involved in the maintenance of the adult nervous system. BDNF and other neurotrophins are critically involved in long-term potentiation(LTP). BDNF-mediated LTP is induced postsynaptically. BDNF has trophic effects on serotonergic(5-HT) neurons in the central nervous system. BDNF has an essential maintenance function in the regulation of anxiety-related behavior and in food intake through central mediators in both the basal and fasted state. It plays a role in treating breathing disorders such as respiratory insufficiency after spinal injury. The mature form of BDNF is identical in all mammals examined, and the gene encoding horse equine BDNF to chromosome 11, band p13.

Product Categories/Family for BDNF elisa kit

Neuroscience; Neurology Process; Growth and Development; neurotrophins; Neuroscience; Neurodegenerative Disease; huntington's disease; Alzheimer's Disease; Cardiovascular; Atherosclerosis; Diabetes Associated; Thrombosis; Metabolism; Types of Disease; dia

NCBI and Uniprot Product Information

• NCBI GI #: 219842288
• NCBI GeneID: 627
• NCBI Accession #: NP_001137277.1
• NCBI GenBank Nucleotide #: NM_001143805.1
• UniProt Accession #: P23560; Q598Q1; Q6DN19; Q6YNR2; Q6YNR3; Q9BYY7; Q9UC24; A7LA85; A7LA92; D3DQZ2
• Molecular Weight: 27818 MW
• NCBI Official Full Name: brain-derived neurotrophic factor isoform a preproprotein
• NCBI Official Synonym Full Names: brain derived neurotrophic factor
• NCBI Official Symbol: BDNF
• NCBI Official Synonym Symbols: ANON2; BULN2
• NCBI Protein Information: brain-derived neurotrophic factor
• UniProt Protein Name: Brain-derived neurotrophic factor
• Protein Family: BDNF/NT-3 growth factors receptor
• UniProt Gene Name: BDNF
• UniProt Synonym Gene Names: BDNF

NCBI Description

This gene encodes a member of the nerve growth factor family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protein. Binding of this protein to its cognate receptor promotes neuronal survival in the adult brain. Expression of this gene is reduced in Alzheimer's, Parkinson's, and Huntington's disease patients. This gene may play a role in the regulation of the stress response and in the biology of mood disorders. [provided by RefSeq, Nov 2015]

Uniprot Description

During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.